Page 39 - 南京医科大学自然版

第45卷第3期 南京医科大学学报（自然科学版）
2025年3月 Journal of Nanjing Medical University（Natural Sciences） ·327 ·

·基础研究·

广金钱草总黄酮抑制M1型巨噬细胞极化减少小鼠肾草酸钙结

石形成

宗益平 ，张俊麒 ，王子杰 ，韩 鹏 ，张 炜 ，鲁 佩 3*
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宜兴市人民医院泌尿外科，江苏 宜兴 214222；南京医科大学第二附属医院泌尿外科，江苏， 南京 210003；南京医科大
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学第一附属医院泌尿外科，江苏 南京 210029
［摘 要］ 目的：探究广金钱草总黄酮（total flavonoids of Desmodium styracifolium，TFDS）对肾草酸钙结石形成的作用及可能机
制。方法：构建选择性巨噬细胞mTOR复合物2的RPTOR独立伴侣（RPTOR independent companion of mTOR complex 2，Rictor）
基因敲除小鼠（Rictor Cre ）及其对照组小鼠（Rictor Cre ）的肾草酸钙结石模型，并给予TFDS干预。收集各组小鼠肾组织行
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HE染色、免疫组织化学荧光染色、流式细胞术等检查，探讨TFDS对小鼠肾草酸钙结石形成的影响；原代提取上述两种小鼠的
骨髓来源巨噬细胞（bone marrow derived macrophage，BMDM），分别给予TFDS干预，通过PCR、细胞荧光染色、流式细胞术等检
探究上述机制。结果：Rictor Cre 组小鼠的肾组织中肾草酸钙结石数目显著多于Rictor Cre 组，并伴随M1型巨噬细胞极化明
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显增多；TFDS 干预两组小鼠后，TFDS+Rictor Cre 组的肾组织中肾草酸钙结石及 M1 型巨噬细胞极化均显著降低，而 TFDS+
Rictor Cre 组的肾草酸钙结石形成较Rictor Cre 组无明显变化。细胞实验表明，Rictor敲除后的BMDM出现了显著的M1型巨
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噬细胞极化，TFDS干预未敲除Rictor的BMDM组中的M1型巨噬细胞极化明显减少；而TFDS干预后Rictor敲除的BMDM极化较干
预前无显著变化。结论：TFDS有效且安全地减少肾草酸钙结石的形成可能与抑制Rictor调控的M1型巨噬细胞极化有关。
［关键词］ 巨噬细胞极化；肾草酸钙结石；Rictor；广金钱草总黄酮
［中图分类号］ R285.5 ［文献标志码］ A ［文章编号］ 1007⁃4368（2025）03⁃327⁃07
doi：10.7655/NYDXBNSN240984
The total flavonoids of Desmodium styracifolium reduces M1 via inhibiting the formation of
renal calcium oxalate stones in mice macrophage polarization
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ZONG Yiping ，ZHANG Junqi ，WANG Zijie ，HAN Peng ，ZHANG Wei ，LU Pei 3*
Department of Urology，Yixing People’s Hospital，Yixing 214222；Department of Urology，the Second Affiliated
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Hospital of Nanjing Medical University，Nanjing 210003；Department of Urology，the First Affiliated Hospital of
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Nanjing Medical University，Nanjing 210029，China
［Abstract］ Objective：To investigate the effect and possible mechanism of total flavonoids of Desmodium styracifolium（TFDS）on
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the formation of renal calcium oxalate stones. Methods：The selective macrophage Rictor gene knockout mice（Rictor Cre ）and their
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control group mice（Rictor Cre ）were used to establish renal calcium oxalate stone models，and the TFDS intervention was
administered to the mice. We collected the kidney tissues from these two groups of mice for HE staining，immunohistochemical
fluorescence staining，flow cytometry to explore the effect of TFDS on the formation of renal calcium oxalate stones. Primary bone
marrow⁃derived macrophage（BMDM）from the two types of mice was extracted，and TFDS intervention was administered to investigate
the mechanism through PCR，cell fluorescence staining，flow cytometry. Results：The number of renal calcium oxalate stones of the
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Rictor Cre mice was significantly higher than that in the Rictor Cre mice，accompanied by a significant increase in the polarization
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of M1 macrophages. After TFDS intervention in two groups of mice，the renal tissue of the TFDS + Rictor Cre group showed a
significant decrease in renal calcium oxalate stones and M1 macrophage polarization，while the formation of renal calcium oxalate
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stones in the TFDS+Rictor Cre group showed no significant changes compared with the Rictor Cre group. Cell experiments showed
［基金项目］ 无锡市卫生健康委科研项目（M202241）
通信作者（Corresponding author），E⁃mail：lupei_nanjing@163.com（ORCID：0009⁃0006⁃8975⁃5355）
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