Page 25 - 《南京医科大学学报（自然科学版）》2025年第9期

第45卷第9期 张 鸽，陈 昕，顾婧瑶，等. circ_0003633对肺腺癌细胞奥希替尼耐药的作用研究［J］.
2025年9月 南京医科大学学报（自然科学版），2025，45（9）：1229-1241 ·1237 ·

A
H1975/OR
HCC827/OR
5 4 * * * si⁃NC 4 * si⁃NC
Relative expression of mRNA 3 2 * * * * * * * si⁃circ_0003633⁃2# Relative expression of mRNA 3 2 si⁃circ_0003633⁃2#
si⁃circ_0003633⁃1#
si⁃circ_0003633⁃1#
*
*
*
*
0 1 1 0
ERBB3 GSTA1 OBSCN NPR1 FOS NOTCH ALDOC ERBB3 GSTA1 OBSCN NPR1 FOS NOTCH ALDOC

B
HCC827/OR H1975/OR
si⁃circ_0003633⁃1# si⁃circ_0003633⁃1#
si⁃circ_0003633⁃2#
si⁃circ_0003633⁃2#
si⁃NC si⁃NC
ALDOC 39 kDa ALDOC 39 kDa

Tubulin 55 kDa Tubulin 55 kDa

C HCC827/OR H1975/OR
si⁃circ_0003633⁃1# si⁃circ_0003633⁃1#

si⁃NC si⁃NC

39 kDa 39 kDa
ALDOC ALDOC
Tubulin 55 kDa Tubulin 55 kDa

D HNRNPA1 vs. ALDOC，501 samples（LUSC） HNRNPA1 vs. ALDOC，526 samples（LUAD）
log2（FPKM+0.01） ［ 8 6 Data Source；starBase v3.0 project （FPKM+0.01） log2 8 6 Data Source；starBase v3.0 project

Regression（y=0.284 5x+1.028 2）
Regression（y=0.468 8x+0.967 0）
r=0.094，P⁃value=3.20e-02
r=0.182，P⁃value=4.03e-05
Expression level： 4 2 0 Expression level： 4 2 0

ALDOC， -2 5 HNRNPA1，Expression level： 9 ALDOC， -2 5 HNRNPA1，Expression level：
8
6
5.5 6.0 6.5 7.0 7.5 8.0 8.5
7
log2 （FPKM+0.01） log2 （FPKM+0.01）
E F
1.5 HCC827/OR 1.5 H1975/OR HCC827/OR H1975/OR
*
**
Relative expression of ALDOC 1.0 Relative expression of ALDOC 1.0 si⁃NC si⁃hnRNPA1⁃1# si⁃hnRNPA1⁃2# si⁃NC si⁃hnRNPA1⁃1# si⁃hnRNPA1⁃2#
**
*
0.5
0.5
0 0 ALDOC 39 kDa ALDOC 39 kDa
si⁃NC
si⁃NC
si⁃hnRNPA1⁃2#
si⁃hnRNPA1⁃2#
si⁃hnRNPA1⁃1# si⁃hnRNPA1⁃1# Tubulin 55 kDa Tubulin 55 kDa
A：Validation of the top seven differentially expressed genes via qRT⁃PCR. B：Assessment of ALDOC protein expression level alterations using
Western blot analysis. C：Evaluation of ALDOC expression dynamics in stably transfected cell lines. D：In silico prediction of the regulatory association
between hnRNPA1and ALDOC utilizing bioinformatic platforms. E，F：Experimental verification of the hnRNPA1 ⁃ ALDOC regulatory relationship
*
**
through qRT⁃PCR and Western blot assays. P < 0.05 and P < 0.01（n=3）.
图7 circ_0003633与hnRNPA1共同影响ALDOC表达
Figure 7 circ_0003633 and hnRNPA1 jointly affected the expression of ALDOC