Page 23 - 《南京医科大学学报（自然科学版）》2025年第9期

第45卷第9期 张 鸽，陈 昕，顾婧瑶，等. circ_0003633对肺腺癌细胞奥希替尼耐药的作用研究［J］.
2025年9月 南京医科大学学报（自然科学版），2025，45（9）：1229-1241 ·1235 ·

A 50 ** B pc⁃DNA HCC827/OR pc⁃DNA H1975/OR
pc⁃circ_0003633
pc⁃circ_0003633
Relative expression of circ_0003633 30 ** μmol/L 0
40
20
10
0 Osimertinib
pc⁃DNA
pc⁃DNA
pc⁃circ_0003633 pc⁃circ_0003633 1.0 μmol/L

H1975/OR HCC827/OR
400 ** ** pc⁃DNA
pc⁃circ_0003633
Number of colonies 300 ** ** pc⁃DNA+1.0 μmol/L Osimertinib
pc⁃circ_0003633+1.0 μmol/L Osimertinib
200
100

0
HCC827/OR H1975/OR pc⁃DNA
C HCC827/OR H1975/OR pc⁃circ_0003633
pc⁃DNA+1.0 μmol/L Osimertinib
pc⁃DNA pc⁃circ_0003633 pc⁃DNA pc⁃circ_0003633
pc⁃circ_0003633+1.0 μmol/L
Osimertinib
μmol/L 300 * * * *
Osimertinib 0 Number of cells 200

1.0 μmol/L 100

0
HCC827/OR H1975/OR
A：qRT⁃PCR was used to detect the overexpression efficiency of circ_0003633. B：The colony formation assay indicated that the proliferation ability
*
of cells overexpressing circ_0003633 was enhanced. C：After overexpression of circ_0003633，the cell migration ability was increased. P < 0.05 and
**
P < 0.01（n=3）.
图4 过表达circ_0003633后对耐药细胞的影响
Figure 4 Effect of overexpression of circ_0003633 on drug⁃resistant cells

A B HCC827/OR C HCC827/OR
si⁃circ_0003633⁃2#
Marker NC circ_0003633 NC circ_0003633
Marker
180 kDa si⁃circ_0003633⁃1#
180 kDa 130 kDa
130 kDa 100 kDa si⁃NC
100 kDa
70 kDa 34 kDa
70 kDa hnRNPA1
55 kDa Tubulin 55 kDa
55 kDa
40 kDa 40 kDa H1975/OR
si⁃circ_0003633⁃2#
35 kDa
35 kDa si⁃circ_0003633⁃1#
25 kDa si⁃NC
25 kDa
34 kDa
hnRNPA1
15 kDa
15 kDa
β⁃actin 55 kDa
A：Silver staining result of RNA pull down assay. B：Western blot verification of the result of RNA pull down assay. C：Western blot result showed
that the expression of hnRNPA1 remained unchanged after knockdown of circ_0003633.
图5 circ_0003633与hnRNPA1结合
Figure 5 circ_0003633 binds to hnRNPA1