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第46卷第10期 李 易，商 艳，陆 超. 转录因子ZNF326在急性T淋巴细胞白血病细胞中的致病作用
2026年10月 研究［J］. 南京医科大学学报（自然科学版），2026，46（10）：1492-1498 ·1495 ·
7 细胞出现荧光，说明过表达实验成功（图3A）；RT⁃qPCR
P < 0.001 结果显示，与LV⁃NC组相比，过表达组（LV⁃ZNF326）
Relative expression of ZNF326 mRNA level 5 4 3 2 0.001，图3B）；Western blot 结果进一步证实，敲低组
6
细胞中 ZNF326 的 mRNA 表达水平显著升高（P <
ZNF326蛋白表达水平高于LV⁃NC组（图3C）；CCK⁃8
结果显示：与NC组相比，过表达组细胞的增殖能力提
ZNF326可显著促进T⁃ALL细胞的增殖。
敲低ZNF326抑制T⁃ALL细胞周期并促进细胞
0 1 高（P < 0.000 1，图 3D）。 以 上 结 果 表 明 过 表 达
2.4
Normal（n=337）Tumor（n=135）
凋亡
图1 ZNF326在正常组织和ALL组织中的表达水平
Figure 1 Expression levels of ZNF326 in normal tissues 流式细胞术结果显示，相比NC组，敲低ZNF326
and ALL tissues 后 T⁃ALL 细胞发生了明显的凋亡且将细胞阻滞在
A B C
1.5 *** 1.5 si⁃NC
*** si⁃ZNF326 Jurkat si⁃NC si⁃ZNF326
Relative ZNF326 mRNA expression 1.0 ZNF326 —66 kDa nm） （450 D 1.0 *** ***
—35 kDa
GAPDH
0.5
0.5
0 0
Jurkat si⁃NC si⁃ZNF326 0 h 24 h 48 h 72 h
A：RT⁃qPCR was performed to validate the knockdown efficiency of ZNF326 in Jurkat cells（si⁃ZNF326）. The results showed that the expression
level in the si⁃ZNF326 group was significantly decreased. B：Western blot analysis of ZNF326 protein expression levels was performed to further vali⁃
date the knockdown efficiency. C：The effect of ZNF326 knockdown on the proliferation of Jurkat cells was assessed by CCK⁃8 assay. The results showed
that the proliferative capacity of cells in the knockdown group was significantly reduced. *** P < 0.001（n=3）.
图2 敲低ZNF326抑制T⁃ALL细胞的增殖
Figure 2 Knockdown of ZNF326 inhibited the proliferation of the T⁃ALL cells
A B *** C
（×100） mRNA expression 3 *** Jurkat LV⁃NC LV⁃ZNF326
LV⁃NC 2 ZNF326 —66 kDa
—35 kDa
GAPDH
（×200） 1
A B Relative ZNF326 0 D 8 LV⁃NC
LV⁃ZNF326 Jurkat LV⁃NC LV⁃ZNF326 nm） 6 LV⁃ZNF326 ***
（450 4
D
2
0
0 h 24 h 48 h 72 h
A：Fluorescence images provided preliminary evidence of successful overexpression. B：RT⁃qPCR was performed to validate the overexpression
efficiency of ZNF326 in Jurkat cells. The results showed that ZNF326 expression was significantly upregulated. C：Western blot analysis of ZNF326
protein levels further confirmed the overexpression efficiency. D：CCK⁃8 assay showed that the proliferative capacity of cells in the overexpression group
***
was significantly increased， P < 0.01（n=3）.
图3 过表达ZNF326促进急性T淋巴细胞白血病细胞的增殖
Figure 3 Overexpression of ZNF326 promoted the proliferation of the T⁃ALL cells
