Page 17 - 南京医科大学学报自然科学版

第41卷第11期 南京医科大学学报（自然科学版）
2021年11月 Journal of Nanjing Medical University（Natural Sciences） ·1579 ·

·基础研究·

亲水末端硅烷接枝促进氧化锆表面骨结合的初步证据

洪高英 1，2，3 ，陈 晨 2，3，4 ，谢海峰 1，2，3*
南京医科大学附属口腔医院口腔修复科，江苏 南京 210029；江苏省口腔疾病研究重点实验室，江苏 南京 210029；江
1 2 3
苏省口腔转化医学工程研究中心，江苏 南京 210029；南京医科大学附属口腔医院口腔牙体牙髓科，江苏 南京 210029
4

［摘 要］ 目的：观察接枝不同末端硅烷的氧化锆材料对MC3T3⁃E1小鼠前成骨细胞黏附、增殖和分化的影响。方法：通过共
价结合的方式在氧化锆表面接枝不同末端基团的硅烷，X射线光电子能谱（X⁃ray photoelectron spectroscopy，XPS）、扫描电子显
微镜（scanning electron microscope，SEM）、X射线衍射（X⁃ray diffraction，XRD）分析检测各组氧化锆表面的理化表征，通过激光
共聚焦显微镜观察MC3T3⁃E1细胞在24 h时的黏附及形态，在培养1、3、5 d后通过CCK⁃8法评价各组细胞增殖水平，使用碱性
磷酸酶试剂盒检测成骨细胞培养 7、14 d 后的分化情况。结果：XPS 结果显示 4 种不同末端硅烷均成功接枝到氧化锆表面；
SEM、XRD结果提示碱热及硅烷处理不破坏氧化锆表面完整性及晶相结构；MC3T3⁃E1细胞在接枝亲水的氨基、巯基末端硅烷
的氧化锆表面黏附情况好，增殖活性高，且碱性磷酸酶的表达较未处理组高，而疏水末端甲基、乙烯基末端硅烷对细胞增殖无明显
影响，对细胞分化起抑制作用。结论：接枝亲水末端硅烷的氧化锆表面对小鼠成骨细胞的黏附、增殖和分化均有促进作用。
［关键词］ 硅烷；氧化锆种植体；表面改性；骨结合
［中图分类号］ R783.1 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）11⁃1579⁃06
doi：10.7655/NYDXBNS20211103

The surface of zirconia grafted with hydrophilic end silanes promotes osteogenesis
HONG Gaoying 1，2，3 ，CHEN Chen 2，3，4 ，XIE Haifeng 1，2，3*
1 Department of Prosthodontics，the Affiliated Stomatological Hospital of Nanjing Medical Universing，Nanjing
210029；Jiangsu Province Key Laboratory of Oral Diseases，Nanjing 210029；Jiangsu Province Engineering
3
2
4
Research of Stomatological Translational Medicine，Nanjing 210029；Department of Endodontics，the Affiliated
Stomatological Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：This study aims to investigate the influence of silanes with different end groups grafting on the zirconia
surface on the adhesion，proliferation and differentiation of osteoblast． Methods：A self⁃assembled monolayer was formed on the
surface of zirconia by covalent bonding. After hydroxylation，zirconia surface was grafted with different terminal groups of silanes，and
then mouse preosteoblasts are cultured on the surface. X⁃ray photoelectron spectroscopy（XPS），scanning electron microscope（SEM）
and X ⁃ ray diffraction（XRD）analysis were used to detect the physical and chemical characteristics of the zirconia surface. The
adhesion and morphology of MC3T3⁃E1 cells were observed by laser confocal microscope after 24 h culture. After 1，3 and 5 days，the
cell proliferation level of each group was evaluated by the CCK⁃8 assay，and the differentiation of osteoblasts after 7 and 14 days of
culture was detected by the alkaline phosphatase kit. Results：XPS results showed that the four different terminal silanes were
successfully grafted to the zirconia surface. SEM and XRD results indicated that alkali heat and silane treatment had no obvious impact
on the topography and crystal phase of zirconia surface. Zirconia grafted with hydrophilic terminal amino⁃ and mercapto⁃ silanes，
presented excellent adhesion and high proliferation activity，and the expression of alkaline phosphatase is higher than that of the
untreated group，while the hydrophobic methyl ⁃ and vinyl ⁃ silanes has no obvious effect on cell proliferation，and inhibit cell
differentiation. Conclusion：Zirconia modified with hydrophilic end groups（such as mercapto and amino）of silanes can promote cell
adhesion，proliferation and differentiation.
［Key words］ silane；zirconia implant；surface modification；osteogenesis
［J Nanjing Med Univ，2021，41（11）：1579⁃1584］
［基金项目］ 国家自然科学基金（81970927）；江苏高校优势学科建设工程资助项目（2018⁃87）；江苏省卫生健康委医学科研项
目（M2020066）
∗
通信作者（Corresponding author），E⁃mail：xhf⁃1980@126.com