Page 130 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第2期
·280 · Journal of Nanjing Medical University（Natural Sciences） 2021年2月

·技术方法·

一种简易、多用途的小鼠固定装置

秦豪男 ，刘星婧 ，丁其瑞 ，马 成 ，魏易凡 ，任永信 1*
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南京医科大学第一附属医院骨科，江苏 南京 210029；东南大学附属中大医院内分泌科，江苏 南京 210009
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［摘 要］ 目的：介绍一种简易、多用途的小鼠固定装置的制作和使用方法。方法：选用废弃的50 mL离心管，按设计方案进
行切割、组装制成简易小鼠固定装置。选取60只小鼠，随机分为2组，对照组采用传统固定方法，研究组则采用本装置固定，先
后对小鼠进行尾静脉注射、断尾采血、膝关节腔内注射，观察比较两组操作时间和成功率等指标。结果：单个50 mL离心管仅
重约 12 g，制作时间约 15 min。对照组和研究组小鼠尾静脉注射时间分别是（32.10±1.97）s 和（30.27±2.20）s，成功率均为
100%，断尾采血时间分别是（30.10±1.73）s 和（28.67±1.55）s，成功率也均为 100%，两组在操作时间上差异均具有统计学意义
（P＜0.01），而在成功率方面均无统计学差异（P＞0.05）。两组膝关节腔内注射时间分别是（40.77±2.73）s和（38.73±2.16）s，成
功率分别为83.33%和95.00%，两组在操作时间及成功率方面差异均具有统计学意义（P＜0.05）。结论：该小鼠固定装置具有
材料易得、制作简单、携带方便、用途多样等优点，为实验小鼠的固定提供了一种新的安全、实用的方法。
［关键词］ 小鼠；固定装置；尾静脉注射；断尾采血；关节腔内注射
［中图分类号］ Q95.336 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）02⁃280⁃06
doi：10.7655/NYDXBNS20210225

A simple and multifunctional device for mouse fixation
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QIN Haonan ，LIU Xingjing ，DING Qirui ，MA Cheng ，WEI Yifan ，REN Yongxin 1*
1 Department of Orthopedics，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029；Department
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of Endocrinology，Zhongda Hospital Affiliated to Southeast University，Nanjing 210009，China
［Abstract］ Objective：This study aims to introduce the manufacture and use instruction for a simple and multifunctional device for
mouse fixation. Methods：Discarded 50 mL centrifuge tubes were collected and processed into the fixation device according to the pre⁃
designed program. A total of 60 mice were randomly and equally assigned into two groups and fixed with traditional methods（control
group）and this fixation device（study group），respectively. Tail vein injections or tail ⁃ clamping blood sampling or intra ⁃ articular
injections were performed. The procedure durations and success rate were recorded. Results：A single 50 ml centrifuge tube weighed
about 12 g and mere 15 minutes were taken to manufact the fixation device. The procedure durations of tail vein injection，tail ⁃
clamping blood sampling and intra⁃articular injections in control and study groups were（32.10±1.97）s and（30.27±2.20）s，（30.10±
1.73）s and（28.67±1.55）s，and（40.77±2.73）s and（38.73±2.16）s，respectively. The data between two groups were significantly
different（P＜0.01）. The success rates of the three procedures in control group study group were 100% and 100 %，100% and 100%，
and 83.33% and 95.00%，respectively. There were significant differences in procedure duration and success rate between two groups
（P＜0.05）. Conclusion：The mouse fixation device possesses the advantages of easily available materials，simple manufacture，
convenient transportation and multiple functions. It provides a new safe and reliable choice for mouse fixation in the experiment and is
worthy to be promoted.
［Key words］ mouse；fixation device；tail vein injection；tail⁃clipping blood sampling；intra⁃articular injection
［J Nanjing Med Univ，2021，41（02）：280⁃285］

实验动物既是生命科学研究的对象和模型，又
［基金项目］ 国家自然科学基金面上项目（81572149）
是生命科学研究的材料和支撑，无论是在基础理论
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通信作者（Corresponding author），E⁃mail：Renyongxinjsph@163.
com 研究、临床试验，还是在新药和生物制品的生产与