Page 110 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第4期
·580 · Journal of Nanjing Medical University（Natural Sciences） 2021年4月

·临床医学·

结肠癌中Nup107的表达与临床病理特征及预后的关系

金 秋 ，石永利 ，林 波 ，王海啸 ，张 晓 ，季润元 1*
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（ 江苏护理职业学院医学基础部，江苏 淮安 223005；南京医科大学附属淮安第一医院病理科，胃肠外科，江苏 淮安
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223300；南京医科大学国家卫生健康委抗体技术重点实验室，江苏 南京 211166）
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［摘 要］ 目的：检测Nup107在结肠癌组织中的表达并探究其与患者临床病理特征及预后的关系。方法：通过Ualcan数据库
和实时定量PCR观察Nup107 mRNA在结肠癌组织中的表达水平；选取南京医科大学附属淮安第一医院结肠癌患者226例，采
用免疫组织化学染色检测Nup107蛋白在结肠癌组织和正常结肠黏膜中的表达水平；结合临床资料分析Nup107表达水平与患
者临床特征及预后的关系；通过基因富集分析初步探讨Nup107在结肠癌中的作用机制。结果：与正常结肠黏膜组织相比，结
肠癌组织中 Nup107 的 mRNA 和蛋白表达水平均显著升高（P＜0.05）；Nup107 表达与肿瘤大小（P=0.004）、淋巴结转移（P=
0.002）、远处转移（P=0.007）、AJCC分期（P=0.003）、Ki67表达（P=0.001）显著相关；Nup107高表达的结肠癌患者总体生存率低
于Nup107低表达的结肠癌患者（P＜0.001）。Nup107在结肠癌中可能参与基础转录因子、错配修复、同源重组、核糖体、剪接
体、RNA降解等途径。结论：Nup107是影响患者预后的独立危险因素，有望为结肠癌患者的诊断和治疗提供新的策略。
［关键词］ Nup107；结肠癌；预后
［中图分类号］ R735.3 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）04⁃580⁃06
doi：10.7655/NYDXBNS20210418

Relationships between Nup107 expression and clinicopathological characteristics and

prognosis in colon cancer
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JIN Qiu ，SHI Yongli ，LIN Bo ，WANG Haixiao ，ZHANG Xiao ，JI Runyuan 1*
1 Department of Basic Medicine，Jiangsu College of Nursing，Huai’an 223005；Department of Pathology，
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3 Department of Gastrointestinal Surgery，the Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical
University，Huai’an 223300；Key Laboratory of Antibody Technology，National Health Commission，Nanjing
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Medical University，Nanjing 211166，China
［Abstract］ Objective： To observe the expression of Nup107 in colon cancer tissues，and explore the relationship of Nup107 with the
clinicopathological characteristics and prognosis of colon cancer patients. Methods：Ualcan database and real⁃time quantitative PCR
were used to observe the expression level of Nup107 mRNA in colon cancer tissues. A total of 226 colon cancer patients from the
Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University were selected. Immunohistochemical staining was used to
detect the expression level of Nup107 protein in colon cancer tissues and normal colon tissues. The relationship of Nup107 expression
level with the clinical characteristics and prognosis was analyzed based on clinical data. Preliminary study on the mechanism of
Nup107 in colon cancer was performed by gene set enrichment analysis. Results：The mRNA and protein expression levels of Nup107
in colon cancer tissues were significantly higher than those in normal colonic mucosal tissues（P < 0.05）. The expression of Nup107
was significantly associated with tumor size（P=0.004），lymph node metastasis（P=0.002），distant metastasis（P=0.007），AJCC stage
（P=0.003），and Ki67 expression（P=0.001）. Colon cancer patients with high level of Nup107 had poorer overall survival than those
with low level of Nup107（P < 0.001）. Nup107 might be involved in basic transcription factors，mismatch repair，homologous
recombination，ribosome，spliceosome，RNA degradation and other pathways in colon cancer. Conclusion：Nup107 is an independent
risk factor for prognosis，and it is expected to provide new strategies for diagnosis and treatment of colon cancer.
［Key words］ Nup107；colon cancer；prognosis
［J Nanjing Med Univ，2021，41（04）：580⁃585］
［基金项目］ 淮安市自然科学研究计划（指导性）项目（HABZ201928）
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通信作者（Corresponding author），E⁃mail： christ7777@163.com