Page 54 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第5期
·684 · Journal of Nanjing Medical University（Natural Sciences） 2021年5月

·临床医学·

ADAM15在支气管哮喘表达及意义

傅 恒，王丽娜，杨月美，郭 帅，姚 欣 *
南京医科大学第一附属医院呼吸与危重症医学科，江苏 南京 210029

［摘 要］ 目的：研究整合素金属蛋白酶 15（a disintegrin and metalloproteinase 15，ADAM15）在支气管哮喘中表达及可能机
制。方法：收集本院32例哮喘患者及同期16例健康对照的临床资料及血清标本，酶联免疫吸附法检测血清ADAM15水平，分
析其与外周血嗜酸性粒细胞数、血清总 IgE 相关性。构建 OVA 诱导的过敏性哮喘小鼠模型，免疫组化观察小鼠肺组织 AD⁃
AM15表达部位和变化。不同细胞因子刺激人支气管上皮细胞系（16HBE），分别用实时定量PCR法和蛋白质免疫印迹法检测
细胞 ADAM15 mRNA 和蛋白表达。结果：与对照组［（398.90±22.13）pg/mL］相比，哮喘患者血清 ADAM15 水平明显增高
［（749.5±132.3）pg/mL，P=0.023］，与血清总IgE水平呈正相关（P=0.001 6，r=0.542 5）。ADAM15主要表达于过敏性哮喘小鼠肺
组织支气管上皮细胞，其表达量较对照组小鼠升高。IL⁃13可上调支气管上皮细胞ADAM15 mRNA及蛋白水平。结论：哮喘中
ADAM15表达增加，可能与IL⁃13介导的哮喘气道炎症有关。

［关键词］ 支气管哮喘；ADAM15；支气管上皮细胞；IL⁃13
［中图分类号］ R562.25 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）05⁃684⁃06
doi：10.7655/NYDXBNS20210508

The expression and significance of ADAM15 in asthma
*
FU Heng，WANG Lina，YANG Yuemei，GUO Shuai，YAO Xin
Department of Respiratory and Critical Care Medicine，the First Affiliated Hospital of Nanjing Medical University，
Nanjing 210029，China

［Abstract］ Objective：This study aims to observe the expression and possible mechanism of a disintegrin and metalloproteinase 15
（ADAM15）in asthma. Methods：The clinical data and serum samples of 32 asthma patients and 16 healthy controls were collected，
enzyme⁃linked immunosorbent assay was used to detect the expression of serum ADAM15，and the correlation of peripheral blood
eosinophils count and percentage，serum total IgE with ADAM15 was analyzed. A mouse model of OVA⁃induced allergic asthma was
constructed，using immunohistochemistry to observe the expression of ADAM15 expression in lung tissue. Real⁃time quantitative PCR
and Western blotting were used to detect the effect of different cytokines stimulation on the expression of ADAM15 in human bronchial
epithelial cell line 16HBE. Results：The level of serum ADAM15 in the asthma group was higher than in the healthy control group
［（749.5±132.3）pg/mL vs.（398.90±22.13）pg/mL，P=0.023］. ADAM15 is mainly expressed in bronchial epithelial cells in mouse lung
tissue. After 24 hours of stimulation of exogenous IL⁃13（10 ng/mL），the mRNA and protein levels of ADAM15 were up⁃regulated.
Conclusion：The expression of ADAM15 is elevated in asthma，and may be related with the IL⁃13⁃mediated airway inflammation.
［Key words］ asthma；ADAM15；bronchial epithelial cell；IL⁃13
［J Nanjing Med Univ，2021，41（05）：684⁃689］

支气管哮喘（简称哮喘）是全球范围内的常见 敏性哮喘作为最常见哮喘类型，由 Th2 型细胞因子
［1］
病和多发病，目前发病率仍有不断上升趋势 。过 ［白细胞介素（interleukin，IL）⁃4、5 和 13］驱动，血嗜
酸性粒细胞增多和免疫球蛋白 E（immunoglobulin
［2］
［基金项目］ 国家自然科学基金（81870039） E，IgE）升高是其主要临床特征 。
∗
通信作者（Corresponding author），E⁃mail：yaoxin@njmu.edu.cn 最近一项全基因组关联研究（genome⁃wide asso⁃