Page 11 - 南京医科大学学报自然科学版

第41卷第6期 袁 逸，戴程婷，李一卉，等. 大宫内发育迟缓新生小鼠胰腺发育转录组分析［J］.
2021年6月 南京医科大学学报（自然科学版），2021，41（06）：785-795 ·789 ·

A B
Hypertrophic cardiomyopathy（HCM） 4 505
Arrhythmogenic right ventricular cardiomyopathy（ARVC） 3 604
Pathways in cancer
Transcriptional misregulation in cancers Num of Genes 2 703
Dopaminergic synapse 1 802
Valine，leucine and isoleucine biosynthsis GeneNumber
Glutamatergic synapse 50 901
Serotonergic synapse 100
150 0
signaling
binding
multiceilular organismal process
membrane part
structural moecule activity
extracellular region part
behavior
translation regulator activity
molecular function regulator
cell part
molecular transducer activity
protein tag
Calcium signaling pathway 200 biological adhesion biological regulation cell aggregation cell killing cellular process detoxification developmental process immune system process growth localization locomotion metabolic process multi⁃organism process reproduction reproductive process response to stimulus rhythmic process single⁃organism process cell cell junction extracellular matrix extracellular region macromolecular complex membrane membrane⁃enc
Pathway Proteoglycans in cancer QValue cellular component organization of biogenesis presynaptic process invoived in synaptic transmission extracellular matrix component nucleic acid binding transcnption factor activity transcription factor activity， protein binding
Circadian rhythm
Retrograde endocannabinoid signaling 6e⁃05
Circadian entrainment 4e⁃05
Dilated cardiomyopathy（DCM） 2e⁃05 Biological Process Cellular Component Molecular Function
Cholinergic synapse
Phosphatidylinositol signaling system
Phospholipase D signaling pathway
Gastric acid secretion
Vascular smooth muscle contraction
Morphine addiction
0.2 0.3 0.4 0.5 0.6
Rich Factor
A：差异LncRNA Antisense作用靶基因KEEG 分析，纵轴表示通路名称，横轴表示富集指数，富集指数越大，表示富集程度越大，点的大小
表示富集在此通路中差异表达的基因个数，点的颜色代表不同的P值；B：差异LncRNAs Antisense 作用靶基因的GO富集分析，图中横轴表示
GO富集条目，纵轴表示基因数。
图2 差异LncRNA Antisense作用靶基因KEEG和GO富集分析
Figure 2 The KEEG and GO enrichment analysis of differentially expressed LncRNA antisense target genes
A B
6 745
Spliceosome
Ribosome 5 396
Huntington’s disease
Salmonella infection Num of Genes 4 047
Alcoholism 2 698
Oxidative phosphorylation
Rheumatoid arthritis GeneNumber 1 349
Longevity regulating pathway⁃mammal 100
translation regulator activity
behavior
macromolecular complex
multi⁃organism process
molecular function regulator
multiceilular organismal process
reproduction
cell part
Bacterial invasion of epithelial cells 200 0 biological adhesion biological regulation cell aggregation cell killing cellular process detoxification growth localization locomotion metabolic process reproductive process response to stimulus rhythmic process signaling cell junction cell extracellular matrix extracellular region membrane membrane part nucleoid organelle organelle part other organism other organism part supramolecular fiber synapse synapse pa
nucleic acid binding transcnption factor activity
signal transducer activity
structural moecule activity
Pathway Epstein⁃Barr virus infection QValue cellular component organization of biogenesis developmental process immune system process presynaptic process invoived in synaptic transmission single⁃organism process extracellular matrix component extracellular region part membrane⁃enclosed lumen chemoattractant activity chemorepellent activity electron carrier activity molecular transducer activity transcription factor activity， protein binding
Proteoglycans in cancer
6e⁃05
Non⁃alcoholic fatty liver disease（NAFLD） 4e⁃05
Protein processing in endoplasmic reticulum 2e⁃05
Parkinson’s disease
Biological Process Cellular Component Molecular Function
RNA polymerase
Pathways in cancer
Lysosome
Alzheimer’s disease
Pyrimidine metabolism
Bladder cancer
0.20 0.25 0.30 0.35
Rich Factor
A：差异LncRNA Cis作用靶基因KEEG 分析；B：差异LncRNAs Cis作用靶基因GO分析。
图3 差异LncRNA Cis作用靶基因KEEG、GO富集分析
Figure 3 The KEEG and GO enrichment analysis of differentially expressed LncRNA Cis target genes
（图4A）。按照GO分析基因表达数目可知：BP中前 mRNA，2 000个差异表达mRNA，其中1 711个上调，
5位是“cellular process”“single⁃organism process”“bi⁃ 289个下调，部分差异表达mRNA如表3所示。
ological regulation”“metabolic process”“response to 2.2.2 差异 mRNA GO 功能与 KEEG 信号通路富集
stimulus”，CC 前 5 位 是“cell”“cell part”“organelle” 分析
“membrane”“membrane part”，MF 前 5 位是“binding” mRNA 差异表达 KEEG 和 GO 信号通路富集分
“catalytic activity”“transporter activity”“molecular 析见图 5。按照 KEEG 信号通路富集分析中基因数
transducer activity”“signal transducer activity”（图4B）。 量比，前 5 位分别是“PI3K⁃Akt signaling pathway”
2.2 mRNA相关分析 （111 个基因）、“Endocytosis”（104 个基因）“MAPK
2.2.1 mRNA表达差异情况 signaling pathway”（100 个 基 因）、“Rap1 signaling
在正常、IUGR 新生鼠中共检测出 66 652 个 pathway”（86 个基因）、“FoxO signaling pathway”（64