Page 62 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第7期
·992 · Journal of Nanjing Medical University（Natural Sciences） 2021年7月

·基础医学·

长链非编码RNA LINC01197调节胃癌进展的机制研究

马 佩 ，方 圆 ，查全斌 ，束永前 ，田声望 1*
1，2
1
1，2
2
江苏大学附属金坛医院肿瘤科，江苏 常州 213200；南京医科大学第一附属医院肿瘤科，江苏 南京 210029
1 2
［摘 要］ 目的：探究长链非编码RNA LINC01197在胃癌中的表达以及调节胃癌细胞进展的相关分子机制。方法：基于GE⁃
PIA数据库分析胃癌组织中LINC01197的表达。培养人胃黏膜上皮细胞（GES⁃1）和人胃癌细胞株（SGC⁃7901、BGC⁃823、MGC⁃
803、AGS），实时荧光定量 PCR（qPCR）法检测 LINC01197 表达，采用短发夹 RNA 转染 SGC⁃7901 和 BGC⁃823 细胞干扰
LINC01197的表达，CCK⁃8、克隆形成实验检测胃癌细胞增殖，Transwell实验检测胃癌细胞侵袭迁移，裸鼠皮下成瘤实验检测胃
癌细胞体内增殖。转录组测序技术（mRNA⁃seq）检测差异基因。qPCR、免疫印迹法分析 SERPINA1 相对表达水平。结果：
LINC01197在胃癌组织和胃癌细胞株中表达上调（P < 0.05）；敲低LINC01197抑制胃癌细胞的增殖、侵袭和迁移（P < 0.05），并
且抑制裸鼠肿瘤增殖水平（P < 0.01）；LINC01197敲低后SERPINA1表达量降低（P < 0.05）。结论：LINC01197在胃癌组织和细
胞中高表达，下调LINC01197可能通过抑制SERPINA1的表达从而抑制胃癌细胞增殖、侵袭和迁移。
［关键词］ 胃癌；LINC01197；增殖；侵袭；迁移
［中图分类号］ R735.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）07⁃992⁃08
doi：10.7655/NYDXBNS20210709

Long non⁃coding RNA LINC01197 regulates progression in gastric cancer
1，2 2 1 1，2 1*
MA Pei ，FANG Yuan ，ZHA Quanbin ，SHU Yongqian ，TIANG Shengwang
1 Department of Oncology，Affiliated Jintan Hospital of Jiangsu University，Changzhou 213200；Department of
2
Oncology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective： To investigate the expression and role of long non⁃coding RNA LINC01197 in gastric cancer. Methods：The
expression of LINC01197 in gastric cancer tissues and paracancerous tissues was analyzed based on GEPIA database. Human gastric
epithelial cell line（GES ⁃ 1）and different gastric cancer cell lines（SGC ⁃ 7901，BGC ⁃ 823，MGC ⁃ 803，and AGS）were cultured
respectively，and the expression of LINC01197 was examined by real⁃time fluorescent quantitative PCR（qPCR）. Cell lines SGC⁃7901
and BGC⁃823 were transfected with short⁃hairpin RNA targeting LINC01197（shLINC01197），CCK⁃8 assays and colony formation
assays were carried out to evaluate cell proliferation，transwell assays was performed to evaluate the capability of invasion and
migration. Nude mice were implanted with SGC⁃7901 and BGC⁃823 subcutaneously to evaluate tumor cell growth in vivo. mRNA⁃seq
assay was used to screen the differential expressed genes after LINC01197 knockdown. The expression of SERPINA1 was detected by
qPCR and Western blot. Results：The expressions of LINC01197 in gastric cancer tissues and cell lines were higher than those in
paracancerous tissues and GES ⁃ 1 cells respectively（P < 0.05）. The cell proliferation，invasion and migration were significantly
suppressed after LINC01197 knockdown in gastric cancer cell lines（P < 0.05）. The tumor growth in vivo was significantly inhibited
after LIN01197 knockdown（P < 0.01）. The knockdown of LINC01197 substantially decreased the expression of SERPINA（P < 0.05）.
Conclusion：LINC01197 was up ⁃ regulated in gastric cancer tissues compared to their paired paracancerous tissues and in gastric
cancer cell lines compared to GES⁃1. Down⁃regulation of LINC01197 would inhibit cell proliferation，invasion and migration capability
in gastric cancer cells through partly inhibiting the expression of SERPINA1.
［Key words］ gastric cancer；LINC01197；proliferation；invasion；migration

［J Nanjing Med Univ，2021，41（07）：992⁃998，1014］

［基金项目］ 国家自然科学基金（81802381）
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通信作者（Corresponding author），E⁃mail：tswjtyy@163.com