Page 14 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第1期
· 8 · Journal of Nanjing Medical University（Natural Sciences） 2022年1月

·基础研究·

RREB1对TGF⁃β1诱导系膜细胞增殖的影响

赵雅洁，施会敏，何田田，季嘉玲，甘卫华，张爱青 *
南京医科大学第二附属医院儿肾科，江苏 南京 210003

［摘 要］ 目的：探讨ras反应元件结合蛋白1（ras responsive element binding protein 1，RREB1）对转化生长因子⁃β1（transform⁃
ing growth factor⁃β1，TGF⁃β1）诱导大鼠系膜细胞增殖的影响。方法：用 TGF⁃β1 及 RREB1⁃siRNA 转染干预系膜细胞，CCK⁃8
法检测系膜细胞增殖活力，RT⁃PCR 法检测细胞增殖核抗原（proliferating cell nuclear antigen，PCNA）、平滑肌肌动蛋白
α（α⁃smooth muscle actin，α⁃SMA）及RREB1 mRNA水平，Western blot法检测PCNA、α⁃SMA及RREB1蛋白的表达，流式细胞术
检测系膜细胞周期。结果：与对照组相比较，TGF⁃β1干预后CCK⁃8法检测系膜细胞增殖活力增加（P < 0.01），PCNA、α⁃SMA及
RREB1 mRNA 和蛋白水平增加（P < 0.01），细胞周期检测提示 S 期细胞百分率增多，G1 期细胞百分率减少（P < 0.01）；与
TGF⁃β1组相比较，RREB1⁃siRNA转染+TGF⁃β1干预后的系膜细胞增殖活力下降（P < 0.01），PCNA、α⁃SMA及RREB1 mRNA和
蛋白水平下降（P < 0.01），细胞周期提示 S 期细胞百分率降低，G1 期细胞百分率升高（P < 0.01）。结论：TGF⁃β1 通过上调
RREB1的表达诱导系膜细胞增殖。
［关键词］ RREB1；转化生长因子β1；系膜细胞；增殖
［中图分类号］ R725.9 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）01⁃008⁃06
doi：10.7655/NYDXBNS20220102

Effects of RREB1 on mesangial cell proliferation induced by TGF⁃β1

*
ZHAO Yajie，SHI Huimin，HE Tiantian，JI Jialing，GAN Weihua，ZHANG Aiqing
Division of Pediatric Nephrology，the Second Affiliated Hospital of Nanjing Medical University，Nanjing 210003，
China

［Abstract］ Objective：To investigate the effects of ras responsive element binding protein 1（RREB1）on mesangial cell proliferation
induced by transforming growth factor⁃β1（TGF⁃β1）. Methods：Mesangial cells were cultured in vitro，and transfected with TGF⁃β1
and RREB1⁃siRNA. The proliferation activity of mesangial cells were detected by CCK⁃8. Proliferating cell nuclear antigen（PCNA），
α⁃smooth muscle actin（α⁃SMA）and RREB1 mRNA levels were detected by RT⁃PCR. The expression levels of PCNA，α⁃SMA and
RREB1 proteins were detected by Western blot. Cell cycles of mesangial cells were detected by flow cytometry. Results：Compared
with the control group，proliferation activity of mesangial cells in the TGF⁃β1 group was significantly increased（P < 0.01）. The mRNA
and protein levels of PCNA，α⁃SMA and RREB1 levels in cells were significantly increased（P < 0.01）. The cell cycle indicated that
the percentage of S phase cells increased and the percentage of G1 phase cells decreased（P < 0.01）. In RREB1⁃siRNA+TGF⁃β1
group，proliferation activity of mesangial cells was significantly decreased compared with TGF⁃β1 group（P < 0.01）. The expressions of
PCNA，α⁃SMA and RREB1 levels in cells were significantly decreased（P < 0.01）. The cell cycle indicated that the percentage of S
phase cells decreased and the percentage of G1 phase cells increased（P < 0.01）. Conclusion：TGF ⁃ β1 induces mesangial cells
proliferation by up⁃regulating RREB1 expression.
［Key words］ RREB1；TGF⁃β1；mesangial cell；proliferation
［J Nanjing Med Univ，2022，42（01）：008⁃013］

［基金项目］ 国家自然科学基金（81970664）；江苏省自然科学基金（BK20191082）；江苏省妇幼健康科研项目（F201855）；
“789”卓越人才培养计划（789ZYRC202080119，789ZYRC202090251）
∗
通信作者（Corresponding author），E⁃mail：njaiqing@njmu.edu.cn