Page 61 - 南京医科大学学报自然科学版

第42卷第4期 南京医科大学学报（自然科学版）
2022年4月 Journal of Nanjing Medical University（Natural Sciences） ·511 ·

·临床研究·

人类抗原R在不同分期糖尿病肾病患者中的差异

董佳馨，黄智敏，邢昌赢 *
南京医科大学第一附属医院肾内科，江苏 南京 210029

［摘 要］ 目的：探讨人类抗原 R（human antigen R，HuR）在不同分期糖尿病肾病患者中的差异。方法：选取 2014 年 1 月—
2019年5月在南京医科大学第一附属医院经肾活检明确诊断的糖尿病肾病（diabetic kidney disease，DKD）患者165例以及肾脏
恶性肿瘤患者16例。免疫组化法检测肾组织中HuR的表达水平。根据DKD病理分期进行分组，采用非参数检验比较各组患
者HuR表达的差异。对HuR表达水平与临床指标的关系进行Spearman相关分析。结果：与肿瘤患者癌旁正常肾脏组织相比，
HuR在DKD患者肾脏中表达增高，差异有统计学意义（P < 0.05）。HuR表达大多数位于肾小管上皮细胞，主要在细胞核内表
达，部分在细胞质内表达。HuR在不同分期的DKD患者肾组织中表达有差异，其中Ⅱb期与Ⅲ期、Ⅱb期与Ⅳ期、Ⅲ期与Ⅳ期之
间的差异有统计学意义（P < 0.05），而Ⅱa期与Ⅱb期之间差异无统计学意义。DKD患者HuR表达与年龄、血清肌酐、尿素氮、
血尿酸、C反应蛋白、甲状旁腺激素呈正相关，与估算的肾小球滤过率、血红蛋白、糖化血红蛋白、1，25羟维生素D3呈负相关。
结论：DKD病理分型在Ⅱb以上者HuR表达与病理分期呈正相关。HuR的表达与肾功能相关临床指标具有相关性。
［关键词］ 糖尿病肾病；人类抗原R；纤维化
［中图分类号］ R587.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）04⁃511⁃08
doi：10.7655/NYDXBNS20220408

The difference of human antigen R in different stages of diabetic kidney disease

*
DONG Jiaxin，HUANG Zhimin，XING Changying
Department of Nephrology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：To explore the difference of human antigen R（HuR）expression in patients with diabetic kidney disease
（DKD）at different stages. Methods：A total of 165 DKD patients diagnosed by renal biopsy admitted to the First Affiliated Hospital of
Nanjing Medical University from January 2014 to May 2019 were enrolled，and 16 patients with renal carcinoma were included. The
expression of HuR in the kidney was detected by immunohistochemistry. According to pathological classification of diabetic kidney
disease，the expressions of HuR in each stage were compared by the Kruskal⁃Wallis test. Spearman’s rank correlation was performed
for correlation analysis between HuR level and clinical factors. Results：The expression of HuR in the kidney was increased compared
with paracancerous tissues of patients with renal carcinoma. HuR was mainly expressed in the nucleus，and partly in the cytoplasm.
There were significant differences between diabetic kidney disease stage Ⅱb and stage Ⅲ，stage Ⅱb and stage Ⅳ，stage Ⅲ and stage
Ⅳ（P < 0.05），but no difference between stage Ⅱa and stage Ⅱb was observed. HuR expression was positively correlated with age，
serum creatinine，urea nitrogen，serum uric acid，C⁃reactive protein and parathyroid hormone（PTH），and negatively correlated with
estimated glomerular filtration rate（eGFR），hemoglobin，glycosylated hemoglobin and 1，25⁃（OH） 2 ⁃vitamin D3. Conclusion：The
expression of HuR is positively related to the severity of pathological stages（Ⅱb，Ⅲ，Ⅳ）. The expression of HuR is correlated with
clinical indicators of renal function.
［Key words］ diabetic nephropathy；HuR；fibrosis
［J Nanjing Med Univ，2022，42（04）：511⁃517，528］

［基金项目］ 江苏省医学创新团队（CXTDA2017011）
∗
通信作者（Corresponding author），E⁃mail：cyxing62@126.com