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南京医科大学学报（自然科学版）                                  第42卷第5期
               ·632 ·                     Journal of Nanjing Medical University（Natural Sciences）   2022年5月


             ·基础研究·

              高频rTMS对健康大鼠认知功能与运动皮质LTP变化的相关性

              研究



              徐兴俊 ，沈 滢 ，伍           琦 ，朱 奕 ，郭 川 ，戴文骏 ，章天娇 ，王                彤  2*
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               南京医科大学康复医学院，江苏 南京              211166；南京医科大学第一附属医院康复医学科，江苏                 南京   210029
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             ［摘    要］ 目的：探究前额叶高频重复经颅磁刺激（repetitive transcranial magnetic stimulation，rTMS）是否能提高健康大鼠认知
              功能，以及认知功能与运动皮质长时程增强（long⁃term potentiation，LTP）样可塑性的相关性。方法：24只SD健康成年大鼠，随
              机分为control 组和rTMS 组（n=12），rTMS 组接受为期4周的高频（10 Hz）rTMS，control 组接受4周假刺激。使用水迷宫检测干
              预前后认知功能的改变；通过TMS结合肌电图运动诱发电位（motor evoked potential，MEP）评价干预前后运动皮质（M1区）LTP
              样可塑性的改变；通过Western blot检测干预后大鼠M1区NR1、NR2A、NR2B的蛋白表达改变。结果：前额叶高频rTMS可以提
              高健康大鼠空间情景记忆能力（P＜0.05）；前额叶高频rTMS可以增强M1区可塑性，表现为M1区LTP样可塑性增高及NR1和
              NR2A蛋白表达上调（P＜0.05）；且M1区LTP样可塑性的改变与空间情景记忆能力成正相关（P < 0.05）。结论：高频rTMS可改
              善健康大鼠的空间情景记忆及运动皮质LTP样可塑性，且认知功能与运动皮质LTP样可塑性呈正相关。
             ［关键词］ 重复经颅磁刺激；认知功能；可塑性；运动皮质
             ［中图分类号］ R744.8                   ［文献标志码］ A                        ［文章编号］ 1007⁃4368（2022）05⁃632⁃06
              doi：10.7655/NYDXBNS20220505



              Correlation analysis of cognitive improvement and LTP⁃like plasticity in healthy rats after
              rTMS

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              XU Xingjun ，SHEN Ying ，WU Qi ，ZHU Yi ，GUO Chuan ，DAI Wenjun ，ZHANG Tianjiao ，WANG Tong    2*
              1 Faculty of Rehabilitation Medicine，Nanjing Medical University，Nanjing 211166；Department of Rehabilitation
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              Medicine，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China
             ［Abstract］ Objective：This study aims to investigate the contribution of M1 long ⁃ term potentiation（LTP）⁃ like plasticity to the
              improvement of cognitive function by high frequency repetitive transcranial magnetic stimulation at healthy rat’s head. Methods：A
              total of 24 rats were randomly divided into control group and rTMS group（n=12）. The cognitive function was detected by Morris water
              maze. The M1 plasticity was reflected by the motor⁃evoked potential（MEP）before and after rTMS on the primary cortex（M1），the
              protein expressions of NR1，NR2A and NR2B in the M1 region of rats after high frequency rTMS were detected by Western blot.
              Results：After treatment，the spatial episodic memory and M1 plasticity showed significant improvement versus the control group（P＜
              0.05）. High⁃frequency rTMS in the prefrontal cortex can enhance the plasticity of the M1 region，which is characterized by increased
              LTP⁃like plasticity and up⁃regulated NR1 and NR2A protein expression in the M1 region. And the changes of LTP⁃like plasticity in M1
              region were positively correlated with spatial episodic memory ability（P＜0.05）. Conclusion：High⁃frequency rTMS could improve
              spatial episodic memory and cortical LTP ⁃ like plasticity in healthy rats，and the improvement in spatial contextual memory was
              positively correlated with motor cortical plasticity.
             ［Key words］ repetitive transcranial magnetic stimulation；cognitive function；plasticity；primary motor cortex
                                                                            ［J Nanjing Med Univ，2022，42（05）：632⁃637］





             ［基金项目］ 国家自然科学基金（81672258）
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              通信作者（Corresponding author），E⁃mail：wangtong60621@123.com
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