Page 77 - 南京医科大学学报自然科学版

第42卷第5期 南京医科大学学报（自然科学版）
2022年5月 Journal of Nanjing Medical University（Natural Sciences） ·673 ·

·临床研究·

长链非编码RNA TPTEP1在肺腺癌中的表达及作用研究

潘春峰，刘锦源 *
南京医科大学第一附属医院胸外科，江苏 南京 210029

［摘 要］ 目的：探讨长链非编码RNA（long non⁃coding RNA，lncRNA）TPTEP1在肺腺癌中的表达及其作用。方法：收集86对
肺腺癌患者的癌组织及癌旁组织样本、33例肺腺癌患者术前及术后的血浆样本，采用qRT⁃PCR检测TPTEP1 在所收集组织、血
浆样本及肺腺癌细胞株中的表达量。采用慢病毒过表达TPTEP1在肺腺癌细胞株A549中的表达后，利用CCK⁃8、Transwell实
验评估肺腺癌细胞的增殖和侵袭迁移能力。结果：肺腺癌组织的TPTEP1表达量与对照组相比明显降低，并且其表达量与肺
腺癌的肿瘤大小、分化程度、淋巴结转移和TNM分期明显相关。肺腺癌患者术后血浆中的TPTEP1表达量较术前出现了明显
上升。过表达TPTEP1明显抑制了肺腺癌细胞株的增殖、侵袭和迁移能力。结论：TPTEP1在肺腺癌患者癌组织及术前血浆中
的表达量明显降低，并且可以抑制肺腺癌细胞的增殖和转移能力，可能成为肺腺癌诊断治疗可行的潜在靶标。
［关键词］ 肺腺癌；lncRNA；TPTEP1；诊断
［中图分类号］ R734.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）05⁃673⁃06
doi：10.7655/NYDXBNS20220511

The expression of long non⁃coding RNA TPTEP1 and its tumor⁃suppressor function in
lung adenocarcinoma

PAN Chunfeng，LIU Jinyuan *
Department of Thoracic Surgery，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：This stndy aims to investigate the expression and function of long non⁃coding RNA（lncRNA）TPTEP1 in lung
adenocarcinoma. Methods：Eighty⁃six pairs of lung adenocarcinoma patients’tumor and adjacent⁃tumor tissue samples，33 pairs of
preoperative and postoperative plasma samples from lung adenocarcinoma patients were collected，and qRT⁃PCR was used to detect
the expression of TPTEP1. The correlation between TPTEP1 levels and clinical features were analyzed. After using lentivirus to
overexpress the expression of TPTEP1 in lung adenocarcinoma cell lines A549，CCK⁃8 and tranwell experiments were used to evaluate
the proliferation，invasion and migration ability of lung adenocarcinoma cells. Results：The expression of TPTEP1 in lung
adenocarcinoma tissues and cell lines was significantly lower than that in the control group，and its expression was significantly related
to the tumor size，tumor differentiation，lymph metastasis and TNM stage of lung adenocarcinoma. Overexpression of TPTEP1
significantly inhibited the proliferation，invasion and migration of lung adenocarcinoma cells. Conclusion：The expression of TPTEP1
in lung adenocarcinoma tissue and preoperative plasma is significantly reduced，and it could inhibit the proliferation and metastasis of
lung adenocarcinoma cells，which might provide a feasible potential target for the diagnosis and therapy of lung adenocarcinoma.
［Key words］ lung adenocarcinoma；lncRNA；TPTEP1；diagnosis
［J Nanjing Med Univ，2022，42（05）：673⁃678］

肺癌作为世界上发病率及病死率最高的肿瘤 放疗、化疗及靶向治疗等各项疗法取得了长足发
之一，其分型中肺腺癌的发病率持续性升高，已经 展，其预后仍不够理想 。因此，寻找新的生物标志
［2］
［1］
成为占比最高的肺癌病理亚型 。尽管目前手术、 物及治疗靶点具有重要意义。
长链非编码RNA（long non⁃coding RNA，lncRNA）

［基金项目］ 国家自然科学基金青年项目（81902328） 作为一类不具有编码功能的RNA，近年来被证实在
∗ 转录前、转录后等多种途径参与了多种肿瘤的发生
通信作者（Corresponding author），E⁃mail：14118762@qq.com