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第42卷第7期 王 莉,袁 逸,唐 艺,等. 宫内发育迟缓小鼠妊娠期胰岛lncRNA和mRNA的表达谱分析[J].
2022年7月 南京医科大学学报(自然科学版),2022,42(7):921-931 ·925 ·
(2 个基因)(图 3A)。GO 功能注释分析中绿色表示 (18个基因)、单有机体过程(single⁃origanism process)
下调基因,红色表示上调基因,按照参与 GO 富集 (16 个基因)、生物调节(biological regulation)(11 个
条目下调基因的数量排序,生物学途径(biological 基因)、代谢过程(metabolic process)(11个基因)、刺
process,BP)中前 5 位是细胞进程(cellular process) 激反应(response to stimulus)(9 个基因);细胞组分
Significant Differential Gene Heatmaperential Gene Heatmaperential Gene Heatmap
表达上调
A 表达下调 Volcano plot 表达无差异 B Significant DiffSignificant Diff
2
2 GroupGroup
Group
GroupGroup 22 Group Group
30 30 ENSMUST00000145944ENSMUST00000145944
ENSMUST00000145944
IP
ENSMUST00000034566
ENSMUST00000034566ENSMUST00000034566 IPIP IP
ENSMUST00000161523ENSMUST00000161523
ENSMUST00000161523
TCONS_00111341TCONS_00111341
TCONS_00111341
ENSMUST00000078061ENSMUST00000078061 NPNP NP
NP
ENSMUST00000078061
TCONS_00005189 1 11
ENSMUST00000230157
ENSMUST00000230157ENSMUST00000230157
TCONS_00005189TCONS_00005189 1
TCONS_00099908
25 25 TCONS_00099908TCONS_00099908
TCONS_00072215TCONS_00072215
TCONS_00072215
ENSMUST00000174366
ENSMUST00000174366ENSMUST00000174366
TCONS_00009559
TCONS_00009559TCONS_00009559
ENSMUST00000119274
ENSMUST00000119274ENSMUST00000119274
TCONS_00093630TCONS_00093630
TCONS_00093630
TCONS_00093304
TCONS_00093304TCONS_00093304 0
ENSMUST00000171337
ENSMUST00000171337ENSMUST00000171337
TCONS_00065468 0 00
20
TCONS_00065468TCONS_00065468
ENSMUST00000121052ENSMUST00000121052
ENSMUST00000121052
ENSMUST00000109299ENSMUST00000109299
ENSMUST00000109299
-lgFDR −1*log10(FDR) 20 15 ENSMUST00000052441ENSMUST00000052441 −1
ENSMUST00000230445ENSMUST00000230445
ENSMUST00000230445
ENSMUST00000052441
ENSMUST00000217811ENSMUST00000217811
ENSMUST00000217811
TCONS_00091364
TCONS_00091364TCONS_00091364
ENSMUST00000099277ENSMUST00000099277
ENSMUST00000099277
15
ENSMUST00000027726ENSMUST00000027726
ENSMUST00000027726
ENSMUST00000051711 -1
ENSMUST00000166237 −1−1
ENSMUST00000051711ENSMUST00000051711
ENSMUST00000166237ENSMUST00000166237
ENSMUST00000102922
ENSMUST00000102922ENSMUST00000102922
TCONS_00031583TCONS_00031583
TCONS_00031583
TCONS_00045138
TCONS_00028760TCONS_00028760
TCONS_00028760
ENSMUST00000021028ENSMUST00000021028
ENSMUST00000021028
10 10 l l TCONS_00045138TCONS_00045138
TCONS_00119958TCONS_00119958
TCONS_00119958
TCONS_00005657
TCONS_00005657TCONS_00005657
ENSMUST00000111070 -2
ENSMUST00000208199 −2−2
l
l ENSMUST00000111070ENSMUST00000111070 −2
ENSMUST00000208199ENSMUST00000208199
ENSMUST00000099576
l ENSMUST00000099576ENSMUST00000099576
TCONS_00002360
l TCONS_00002360TCONS_00002360
ENSMUST00000111855
l l ENSMUST00000111855ENSMUST00000111855
ENSMUST00000023057
5 5 l l l l l l l l l l ENSMUST00000023057ENSMUST00000023057
TCONS_00071374
TCONS_00071374TCONS_00071374
TCONS_00040486
ENSMUST00000102927
l TCONS_00040486TCONS_00040486
l l l ENSMUST00000102927ENSMUST00000102927
TCONS_00120600
l l l l l TCONS_00120600TCONS_00120600
l ENSMUST00000112095ENSMUST00000112095
ENSMUST00000112095
l l l l l l l l l l l l
ENSMUST00000016681
l l l l l l ENSMUST00000016681ENSMUST00000016681
l l l l TCONS_00031584TCONS_00031584
TCONS_00031584
0 0 ENSMUST00000212319ENSMUST00000212319
ENSMUST00000212319
ENSMUST00000173954
ENSMUST00000173954ENSMUST00000173954
ENSMUST00000095143
ENSMUST00000095143ENSMUST00000095143
TCONS_00113139TCONS_00113139
TCONS_00113139
−4 -4 −2 -2 0 0 2 2 4 4 NP1 NP1 NP1 NP2 NP2 NP2 NP3 NP3 NP3 IP1 IP1 IP1 IP1 IP2 IP2 IP2 IP2 IP3 IP3 IP3 IP3
NP2
NP3
NP1
log2FC
logFC
A:差异表达mRNA的火山图;B:差异表达mRNA的热图,横坐标表示样本,纵坐标表示差异表达的mRNA。
图2 正常孕鼠和IUGR孕鼠差异表达的mRNA
Figure 2 Differentially expressed mRNAs between normal pregnant and IUGR pregnant mice
表2 差异表达的mRNA
Table 2 Differentially expressed mRNAs
ID mRNA名称 log2FC P值 上调或下调
ENSMUST00000016681 Cul4b 010.892 < 0.001 上调
ENSMUST00000095143 Rbm12b2 010.261 < 0.001 上调
ENSMUST00000212319 Galns 010.027 < 0.001 上调
ENSMUST00000023057 Nptxr 009.871 < 0.001 上调
ENSMUST00000112095 Pbrm1 009.840 < 0.001 上调
ENSMUST00000052441 Slc17a5 -11.015 < 0.001 下调
ENSMUST00000051711 Mark2 -10.951 < 0.001 下调
ENSMUST00000078061 Ehmt2 -10.548 < 0.001 下调
ENSMUST00000027726 Cyb5r1 -10.495 < 0.001 下调
ENSMUST00000034566 Dixdc1 0-9.902 < 0.001 下调
up
A Top 10 of Pathway Enrichment B
Level2 GO terms of NP⁃vs⁃IP down
Metabolic pathways GeneNumber 24
2.0
PI3K⁃Akt signaling pathway
2.5 18
Protein processing in endoplasmic reticulum 3.0
Focal adhesion 3.5 Num of Genes 12
4.0
AMPK signaling pathway 6
Lysosome PValue
0.5 0
biological regulation
chemorepellent activity
FoxO signaling pathway cell
0.4 cell part organelle binding
RNA degradation 0.3 cellular process metabolic process organelle part membrane catalytic activity
Ubiquitin mediated proteolysis 0.2 single⁃organismprocess response to stimulus transporter activity
0.1
Nucleotide excision repair
0.0025 0.0050 0.0075 0.0100 0.0125
RichFactor Biological Process Cellular Component Molecular Function
A:差异mRNA的KEGG信号通路富集分析图,图中横轴表示富集指数,纵轴表示通路名称,富集指数越大表示富集程度越高,点的大小表示
富集在此通路中差异表达的基因个数,点的颜色代表不同的P值;B:差异mRNA的GO功能注释图,横轴表示GO富集条目,纵轴表示基因数目。
图3 正常孕鼠和IUGR孕鼠差异mRNA的KEGG和GO富集分析图
Figure 3 The KEGG and GO enrichment analysis of differentially expressed mRNAs between normal and IUGR pregnant mice