Page 88 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第43卷第2期
·230 · Journal of Nanjing Medical University（Natural Sciences） 2023年2月

·临床医学·

microRNA let⁃7 在 COPD 稳定期男性患者血清中的表达及临床

意义

张哲明 ，吴 艳 ，邸婷婷 ，范晓东 ，郁 震 ，吴 霞 ，王春新 ，邵耀明 ，杨 玥 ，卞 涛 1*
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南京医科大学附属无锡人民医院呼吸与危重症医学科，医学检验科，江苏 无锡 214023
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［摘 要］ 目的：探讨microRNA let⁃7在慢性阻塞性肺疾病（COPD）稳定期男性患者血清中的表达及其临床意义。方法：选取
160例COPD稳定期患者和100例健康志愿者，收集一般临床资料，采集血浆样本并4 ℃离心提取血清；qPCR检测各组血清中
let⁃7a、let⁃7c、let⁃7d 相对表达水平；对 let⁃7 与肺通气功能进行相关性分析；ROC 曲线分析 let⁃7 对 COPD 的诊断价值。结果：
COPD患者血清let⁃7a、let⁃7c、let⁃7d表达量明显低于不吸烟对照组和吸烟对照组（P＜0.05）；GOLD分组之间血清let⁃7表达量无
明显差异（P＞0.05）；吸烟对照组血清let⁃7的表达量低于不吸烟对照组，但差异无统计学意义（P＞0.05）。经Pearson分析，血
清 let⁃7 表达量与 FEV1/FVC、MEF75%pred、MEF50%pred、MEF25%pred、MMEF75/25%pred 均呈正相关（P＜0.05），其中 let⁃7 与
MMEF75/25%pred 的相关性最强；let⁃7c 与 FEV1%pred 呈正相关（P＜0.05），而 let⁃7a、let⁃7d 与 FEV1%pred 无明显相关性（P＞
0.05）。ROC曲线分析结果显示，let⁃7a、let⁃7c、let⁃7d、联合指标的ROC曲线下面积分别为0.808、0.708、0.761、0.828；联合指标的
诊断效能较好，灵敏度、特异度分别为 83.51%、70.51%。结论：血清 let⁃7 表达水平的降低与 COPD 的发病有关，let⁃7 对轻度
COPD患者有诊断价值，可为临床及时诊断和规范治疗提供参考。
［关键词］ 慢性阻塞性肺疾病；let⁃7；肺通气功能
［中图分类号］ R563 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）02⁃230⁃06
doi：10.7655/NYDXBNS20230212

The expression and clinical significance of microRNA LET ⁃ 7 in serum of stable male
COPD patients

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ZHANG Zheming ，WU Yan ，DI Tingting ，FAN Xiaodong ，YU Zhen ，WU Xia ，WANG Chunxin ，SHAO
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Yaoming ，YANG Yue ，BIAN Tao
1 Department of Respiratory and Critical Care Medicine，Department of Medical Laboratory，Wuxi People’s Hospital
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Affiliated to Nanjing Medical University，Wuxi 214023，China
［Abstract］ Objective：To explore the expression of microRNA let⁃ 7 in the serum of stable male COPD patients and its clinical
significance. Methods：One hundred and sixty COPD patients in stable period and 100 healthy volunteers were selected to collect
clinical data and plasma samples. Serum samples were extracted by centrifugation at 4 ℃. The relative expression levels of let⁃7a，let⁃
7c and let⁃7d in serum of each group were detected by qPCR. Correlation analysis was conducted between let⁃ 7 and lung function
indexes. The ROC curve was used to analyze the diagnostic value of let⁃7 for COPD. Results：qPCR results showed that the serum let⁃
7a，let⁃7c and let⁃7d levels in COPD patients were significantly lower than the control and smoking groups（P < 0.05）. The expression
of let⁃7 in GOLD groups had no significant difference（P > 0.05）. The expression levels of let⁃7 in the serum of the smoking group were
lower than those of the control group，but the differences were not statistically significant（P > 0.05）. Pearson analysis showed that the
serum let⁃7 expression had positively correlation with lung function（FEV1/FVC，MEF75%pred，MEF50%pred，MEF25%pred，MMEF75/25%
pred）（P < 0.05），among which let⁃7 had the strongest correlation with MMEF 75/25%pred；let⁃7c was positively correlated with FEV 1%
pred（P < 0.05），while let⁃7a and let⁃7d were not significantly correlated with FEV 1%pred（P > 0.05）. ROC curve analysis showed that
the area under the ROC curve of let⁃7a，let⁃7c，let⁃7d and combined index were 0.808，0.708，0.761，0.828，respectively，the diagnostic

［基金项目］ 国家自然科学基金（82173472）；无锡卫生健康委科研项目（M202032；2020ZHYB13）
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通信作者（Corresponding author），E⁃mail：btaophd@sina.com