Page 46 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第43卷第4期
·484 · Journal of Nanjing Medical University（Natural Sciences） 2023年4月

·基础研究·

ANKRD1 通过介导上皮细胞间充质转化促进肝细胞肝癌增殖

与转移

朱德明，孔连宝，贾文博，夏金国 *

南京医科大学第一附属医院肝胆中心，中国医学科学院肝移植重点实验室，国家卫生健康委员会活体供肝移植重点实验室，江
苏 南京 210029

［摘 要］ 目的：探究ANKRD1在肝细胞肝癌（hepatocellular carcinoma，HCC）中的表达情况，研究ANKRD1促进HCC 细胞增
殖和转移的功能及其机制。方法：通过实时定量RT⁃PCR（real⁃time quantitative PCR，RT⁃qPCR）与Western blot检测ANKRD1在
肝细胞肝癌及癌旁组织中的相对表达量，临床病理学分析 ANKRD1 表达与 HCC 患者临床特征的相关性。利用慢病毒
感染将肝癌细胞系中的 ANKRD1 敲低或过表达，通过 CCK⁃8、划痕、Transwell、EdU 以及裸鼠皮下成瘤实验在体内体外检
测 ANKRD1 对肝癌细胞增殖与迁移功能的影响。Western blot 技术检测 ANKRD1 对 HCC 细胞上皮细胞⁃间充质转化
（epithelial⁃mesenchymal transition，EMT）的调控作用。结果：ANKRD1在肝癌细胞中表达明显上调，过表达ANKRD1可以促进
肝癌细胞增殖、迁移、侵袭，而 ANKRD1 敲低则会抑制肝癌细胞的增殖与迁移，Western blot 与皮下瘤免疫组化实验表明
ANKRD1可以促进HCC细胞EMT通路激活。结论：ANKRD1在肝癌细胞中表达上调并通过调控EMT通路促进HCC细胞增殖
与迁移，有望成为肝癌治疗的新靶点。
［关键词］ ANKRD1；肝细胞肝癌；上皮细胞⁃间充质转化
［中图分类号］ R735.7 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）04⁃484⁃08
doi：10.7655/NYDXBNS20230406

ANKRD1 promotes proliferation and metastasis of hepatocellular carcinoma by activating
epithelial mesenchymal transition pathway

ZHU Deming，KONG Lianbao，JIA Wenbo，XIA Jinguo *
Hepatobiliary Centre，the First Affiliated Hospital of Nanjing Medical University，Key Laboratory of Liver
Transplantation，Chinese Academy of Medical Sciences，National Health Commission（NHC）Key Laboratory of Living
Donor Liver Transplantation，Nanjing 210029，China

［Abstract］ Objective：This study aims to explore the expression of ANKRD1 in hepatocellular carcinoma（HCC）and investigate the
function and mechanism of ANKRD1 in promoting the proliferation and metastasis of HCC. Methods：The relative expression of
ANKRD1 in hepatocellular carcinoma and adjacent tissues was detected by RT⁃qPCR and Western blot. Clinicopathological analysis
was used to assess the association of ANKRD1 with the clinical feature of HCC patients. ANKRD1 was knocked down or overexpressed
in HCC cell lines by lentiviral infection. The effects of ANKRD1 on the proliferation and migration of HCC cells were detected by CCK⁃8
assay，wound healing assay，transwell assay，EdU assay in vitro and subcutaneous tumor model in vivo. The activity of epithelial⁃
mesenchymal transition（EMT）pathway was examined by Western blot. Results：ANKRD1 was upregulated in HCC. Overexpression of
ANKRD1 promoted the proliferation，migration and invasion of HCC cells，while knockdown of ANKRD1 inhibited the proliferation
and migration of HCC cells. Western blot and immunohisbochemical assay of subcutaneous tumor showed that ANKRD1 could activate
EMT pathway. Conclusion：ANKRD1 is upregulated in HCC and promotes the proliferation and migration of HCC cells by regulating
the EMT pathway，providing a potential therapeutic target for HCC.
［Key words］ ANKRD1；hepatocellular carcinoma；EMT
［J Nanjing Med Univ，2023，43（04）：484⁃491］
［基金项目］ 国家自然科学基金（81871260）
∗
通信作者（Corresponding author），E⁃mail：Kinglexia@163.com