Page 11 - 南京医科大学自然版

第44卷第11期 何晓鑫，高俊英，郭吉超，等. Bmi⁃1基因杂合子缺失对小鼠脑老化的影响［J］.
2024年11月 南京医科大学学报（自然科学版），2024，44（11）：1473-1482 ·1477 ·

A +/- B C +/- D
WT Bmi⁃1 50 WT Bmi⁃1 （mm） 0.5
（g） 40 0.4
Body weight 30 Thickness of skin 0.3
20
0.2
10
0 0.1 0
WT Bmi⁃1 +/- WT Bmi⁃1 +/-

E F G
Width of growth plate （μm） 90
WT Bmi⁃1 +/- WT Bmi⁃1 +/- 150
120

60
30
0
WT Bmi⁃1 +/-
H WT Bmi⁃1 +/- I WT Bmi⁃1 +/- J 0.5
0.4
Bone IOD 0.3
0.2
0.1
0
WT Bmi⁃1 +/-
+/-
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A：Appearance of Bmi⁃1 and WT mice. B：Body weight of Bmi⁃1 and WT mice. C：HE staining of the skin showed cellular structures of Bmi⁃1 +/-
and WT mice. Scale bars=50 μm. D：The skin thickness of Bmi⁃1 +/- and WT mice. E：Masson staining of the skin showed the distribution of total colla⁃
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gen in the skin of Bmi⁃1 and WT mice. Scale bars=50 μm. F：HE staining of long bones showing bone morphology and cellular architecture of Bmi⁃1 +/-
and WT mice. Scale bars=100 μm. G：Comparison of bone growth plate thicknesses between Bmi⁃1 +/- and WT mice. H：Masson staining of long bones
showing total collagen expression in bone of Bmi⁃1 +/- and WT mice. Scale bars=200 μm. I：X⁃ray of long bones of Bmi⁃1 +/- and WT mice. J：X⁃ray mea⁃
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surement of optical density in long bones of of Bmi⁃1 and WT mice，showing the size of bone density（n=4）.
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图1 Bmi⁃1 小鼠与WT小鼠的体重、外貌、皮肤及骨骼分析比较
Figure 1 Comparisons of body weight、appearance、skin、and skeletal analysis between the Bmi⁃1 and WT mice
+/-

A B C D
70 24 50 WT 180 WT
（g） 60 20 （sec） 40 Bmi⁃1 +/- （mm/sec） 150 Bmi⁃1 +/-
50
Alternation 40 Number of arm entries 12 8 Latency 20 * * Speed 90
120
16
30
30
60
20
10
10
0 4 0 0 .. 20 0 ..
WT Bmi⁃1 +/- WT Bmi⁃1 +/- 1 d 2 d 1 d 2 d 3 d 4 d 5 d 1 d 2 d 1 d 2 d 3 d 4 d 5 d
E F
40 20
（sec） 30 * 15 *
Duration 20 Number of crossing 10 5
10
0 0
WT Bmi⁃1 +/- WT Bmi⁃1 +/-
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A：Comparison of the proportions of alternation in Y⁃maze test between the Bmi⁃1 mice and WT mice. B：Comparison of the number of arm entries
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between the Bmi⁃1 mice and WT mice. C：Comparison of the latency between the Bmi⁃1 mice and WT mice in finding target quadrant platform under
+/⁃
both visual and non⁃visual platform condition. D：Comparison of the swimming speed between the Bmi⁃1 mice and WT mice. E：Comparison of the dura⁃
+/⁃
tion time between the Bmi⁃1 mice and WT mice in the target quadrant without platform at the next day after non⁃visual platform training. F：Compari⁃
*
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son of the platform crossing times between the Bmi⁃1 mice and WT mice. Compared with the WT mice，P < 0.05（n=7）.
图2 Bmi⁃1 小鼠学习记忆行为学分析
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Figure 2 Behavioural analysis of learning memory in Bmi⁃1 mice
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