Page 32 - 南京医科大学自然版

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·620 · 南 京 医 科 大 学 学 报 2024年5月

4.0
Up NS⁃WT LPS⁃WT
Down
3.5 No difference HSPA12A 75 kDa

GAPDH 36 kDa
3.0
Hspa12a
2.5
｜lgP｜ 2.0 1.2 *
Relative protein expression of HSPA12A 0.8

1.5 1.0

1.0

0.5 0.6
0.4
0 NS⁃WT LPS⁃WT
-15 -10 -5 0 5 10 15
log2FC
A：Volcano plots showed differentially expressed heat shock protein mRNAs between the CLP group and the sham group. B：Comparison of protein
*
expression levels of HSPA12A in NS and LPS groups，P < 0.05（n=3）.
图2 HSPA12A在内毒素血症小鼠肝组织中表达降低
Figure 2 HSPA12A expression was reduced in livers of endotoxemia mice

WT WT
Hspa12a -/- Hspa12a -/-
A B
***
WT Hspa12a -/- 2 000 *** 1 500 ** *
* **
HSPA12A 75 kDa 1 500
（U/L） 1 000 （U/L）
GAPDH 36 kDa 1 000
ALT AST 500
500

C
WT Hspa12a -/- 0 0
NS LPS NS LPS
WT WT
D Hspa12a -/- E Hspa12a -/-
NS * ***
4 8 **
***
***
3 6 ***
Histology score 2 Inflammatory foci/mm 2 4 2

LPS 1 0

0 -2
NS LPS NS LPS

-/-
A：HSPA12A was not expressed in the liver of Hspa12a mice（n=3）. B：Comparison of ALT and AST level in each group（n=8）. C：Representative
graph of HE staining of hepatic tissue in each group（n=3，Scale bar：50 μm）. D：Comparison of histology score in each group（n=3）. E：Comparison of
***
*
**
2
inflammatory foci/mm in each group，P < 0.05，P < 0.01， P < 0.001（n=3）.
图3 Hspa12a基因敲除加重内毒素血症肝损伤
Figure 3 Hspa12a knock⁃out exacerbated liver injury during endotoxemia