Page 29 - 南京医科大学自然版

第44卷第6期 张丽君，李 琳，斯 岩，等. UBE2T通过JAK⁃STAT通路促进甲状腺乳头状癌细胞增殖［J］.
2024年6月 南京医科大学学报（自然科学版），2024，44（6）：762-768 ·765 ·

A *** B C
P2
P3
P1
4.5 UBE2T N T N T N T 23 kDa 4 3 *** Nthyori 3⁃1 KTC⁃1 B⁃CPAP
TPC⁃1
IHH4
Expression of UBE2T （TPM+1） 4.0 GAPDH N T N T N T 37 kDa Relative UBE2T protein exprssion 2 1 GAPDH *** ** 23 kDa
UBE2T
3.5
P4
37 kDa
P6
P5
3.0
Log2
23 kDa
UBE2T
2.5
2.0
1.5 GAPDH 37 kDa 0 N T Relative UBE2T protein exprssion 8 6 4 ** ***
N T 2
D E ** 0
1.0
Nthyori 3⁃1
B⁃CPAP
5 TPC⁃1 KTC⁃1 IHH4
Survival probability 0.5 3
0.8
4
0.3
UBE2T low expression Exprssion of UBE2T 2
UBE2T high expression 1
P=0.003
0 HR=3.43，95%CI（1.43-8.21）
Number at risk 0
Low expression 194 61 17 5
High expression 158 44 14 5
-1
0 1 355 2 710 4 065 5 420
N0（n=229） N1（n=225）
DFI
A：The analysis of UBE2T expression in paired samples from the TCGA⁃THCA dataset. B：The expression levels of UBE2T in PTC tissue samples
were detected by Western blot（n=6）. N：Adjacent normal tissue；T：Tumor. C：The expression of UBE2T in normal thyroid cells and PTC cell lines（n=
3）. D：Bioinformatics analysis predicting the association between UBE2T expression levels and disease⁃free interval（DFI）in patients with PTC. E：Bio⁃
informatics analysis predicting the association between UBE2T expression levels and lymph node metastasis in patients with PTC through THCA data⁃
**
base. N0：PTC without lymph node metastasis；N1：PTC with lymph node metastasis. P < 0.01 and *** P < 0.001.
图1 UBE2T在PTC中的表达及其与临床特征的关联
Figure 1 The expression of UBE2T in PTC samples and its association with clinical features
A TPC⁃1 KTC⁃1 B TPC⁃1 C
1.0 sh⁃Vehicle sh⁃UBE2T
sh⁃Vehicle sh⁃Vehicle 0.8 sh⁃Vehicle
sh⁃UBE2T
sh⁃UBE2T
sh⁃UBE2T
UBE2T 23 kDa nm） 0.6 *** TPC⁃1
（450 0.4
GAPDH 37 kDa
1.5 sh⁃Vehicle D 0.2
sh⁃UBE2T ** 0 KTC⁃1
Relative UBE2T protein expression 1.0 1.0 0 sh⁃Vehicle 48 72 250 sh⁃Vehicle **
***
24
Time（h）
KTC⁃1
0.5
sh⁃UBE2T
nm） 0.8 sh⁃UBE2T *** 200 **
150
0 0.6 Number of colonies
（450 D 0.4
TPC⁃1 KTC⁃1 100
0.2 50
0
0 TPC⁃1 KTC⁃1
0 24 48 72
Time（h）
A：The validation of UBE2T knockdown efficiency by using Western blot. B：The assessment of the proliferative ability of PTC cells after UBE2T
knockdown by using CCK⁃8 assay. C：The colony formation assay was used to evaluate the clonogenic potential of PTC cells after UBE2T knockdown.
** ***
P < 0.01 and P < 0.001（n=3）.
图2 敲减UBE2T抑制PTC细胞增殖
Figure 2 The inhibition of PTC cell proliferation after UBE2T knockdown