Page 31 - 南京医科大学自然版

第44卷第6期 张丽君，李 琳，斯 岩，等. UBE2T通过JAK⁃STAT通路促进甲状腺乳头状癌细胞增殖［J］.
2024年6月 南京医科大学学报（自然科学版），2024，44（6）：762-768 ·767 ·

A 0.5 JAK STAT signaling pathway B TPC⁃1 KTC⁃1 sh⁃Vehicle sh⁃Vehicle
TPC⁃1
KTC⁃1
Enrichment score 0.4 0 p⁃STAT3 sh⁃Vehicle sh⁃Vehicle 86 kDa 2.5 sh⁃UBE2T 2.5 sh⁃UBE2T
sh⁃UBE2T
sh⁃UBE2T
0.3
0.2
2.0
2.0
0.1
STAT3
1.0
1.0
37 kDa
GAPDH 86 kDa Relative protein expression 1.5 *** Relative protein expression 1.5 ***
Ranked list metric 0.8 Low 0 p⁃STAT3 STAT3 0 p⁃STAT3 STAT3
0.5
0.5
High
1.0
0.6
0.4
0.2
0.0
-0.2
5 000 10 000 15 000 20 000 25 000 30 000
0
Rank in ordered data set
C TPC⁃1 KTC⁃1 D sh⁃UBE2T Colivelin+sh⁃UBE2T sh⁃UBE2T
sh⁃UBE2T sh⁃UBE2T Colivelin+sh⁃UBE2T
1.0 1.0
Colivelin+sh⁃UBE2T Colivelin+sh⁃UBE2T 200 *
nm） 0.8 *** nm） 0.8 ** TPC⁃1 150
（450 0.6 （450 0.6 Number of colonies 100 *
0.4
0.4
D
D
0.2
0 0.2 0 KTC⁃1 50
0 24 48 72 0 24 48 72 0
Time（h） Time（h） TPC⁃1 KTC⁃1
E TPC⁃1 F KTC⁃1
sh⁃UBE2T Colivelin+sh⁃UBE2T sh⁃UBE2T Colivelin+sh⁃UBE2T
（%） 150 TPC⁃1 （%） 150 KTC⁃1
Wound closure rate 50 0 Wound closure rate 50
0 h 100 h 0 100
0
sh⁃UBE2T
sh⁃UBE2T
h Colivelin+sh⁃UBE2T h Colivelin+sh⁃UBE2T
24 24
G Migration H Invasion
sh⁃UBE2T Colivelin+sh⁃UBE2T sh⁃UBE2T Colivelin+sh⁃UBE2T
sh⁃UBE2T sh⁃UBE2T
Colivelin+sh⁃UBE2T Colivelin+sh⁃UBE2T
TPC⁃1 60 TPC⁃1 60
Migration cells per field 40 Invasion cells per field 40

20
20
KTC⁃1 0 TPC⁃1 KTC⁃1 KTC⁃1 0 TPC⁃1 KTC⁃1

A：The gene set enrichment analysis（GSEA）of UBE2T in the TCGA dataset（normalized enrichment score=1.83，P < 0.01，false discovery rate=
0.02）. B：The results of Western blot analysis and quantivication to assess changes in phosphorylation levels of PTC cells（TPC⁃1 and KTC⁃1）after
UBE2T knockdown. C：The CCK⁃8 assay was used to evaluate the proliferative ability of sh⁃UBE2T cells（TPC⁃1 and KTC⁃1）treated with Colivelin.
D：The colony formation assay was used to measure the clonogenic potential of sh⁃UBE2T cells（TPC⁃1 and KTC⁃1）treated with Colivelin. E，F：The
wound healing assay was used to evaluate the migration abilities of sh⁃UBE2T cells treated with Colivelin（E：TPC⁃1；F：KTC⁃1；×40）. G，H：The
transwell assay was used to evaluate the migration（G）and invasion（H）abilities of sh⁃UBE2T cells（TPC⁃1 and KTC⁃1）treated with Colivelin（×100）.
* ** ***
P < 0.05，P < 0.01 and P < 0.001（n=3）.
图4 UBE2T通过激活JAK⁃STAT信号通路促进PTC细胞增殖
Figure 4 The promotion of PTC cell proliferation by UBE2T through activation of the JAK ⁃ STAT signaling pathway