Page 42 - 南京医科大学自然版

第44卷第6期
·778 · 南 京 医 科 大 学 学 报 2024年6月

A 1.5 B （%） 80 E NC AGS OE⁃TCEAL7
Relative mRNA expresson 1.0 *** *** Non⁃GC Relative expresson 60 * 0 h

40
0.5
20
0
GC
0
GES⁃1 AGS HGC⁃27
Non⁃GC GC h 48
HGC⁃27
NC
C AGS HGC⁃27 OE⁃TCEAL7
150 250
Colony numbers 50 Colony numbers 100
200
NC 100 * 150 ** 0 h
50
OE⁃TCEAL7 0 OE⁃TCEAL7 0 OE⁃TCEAL7 h 48 NC
NC
NC

D F （%） 60 OE⁃TCEAL7
NC OE⁃TCEAL7 AGS 40
NC OE⁃TCEAL7 NC **
DAPI 200 OE⁃TCEAL7 Relative migration area 20
Migration 150 ** ** 0 **

AGS EdU Cell numbers per field 100 AGS HGC⁃27
Invasion 0 G NC OE⁃TCEAL7
50
Merge HGC⁃27 Migration Invasion

NC OE⁃TCEAL7 NC
NC OE⁃TCEAL7 250 OE⁃TCEAL7 40
DAPI Migration Cell numbers per field 200 ** ** Number of spherolds cells） 500 30 *
150
20
100
HGC⁃27 EdU Invasion 50 0 （per 10

Migration Invasion 0
NC OE⁃TCEAL7
Merge
H
10 4 Q1 Q2 10 4 Q1 Q2 30
NC APC 3 25.7 APC 3 9.22
40 OE⁃TCEAL7 10 1.41 10 1.17 （%） 20
（%） 30 * CD44 10 2 CD44 10 2 CD44 high /CD24 high cells ratio **
EdU/DAPI 20 * FL4⁃H： 10 1 0 67.4 5.48 FL4⁃H： 10 1 0 82.6 6.98 10
Q4
Q3
Q3
Q4
10
10
10
10 0 10 1 10 2 10 3 10 4 10 0 10 1 10 2 10 3 10 4 0 NC OE⁃TCEAL7
0 FL2⁃H：CD24 PE FL2⁃H：CD24 PE
AGS HGC⁃27
A：qRT⁃PCR detected the expression of genes in GC cells（AGS and HGC⁃27）and GES⁃1. B：Results of the expression of TCEAL7 protein in GC
specimens by IHC（×200）. C，D：Assessment of GC cell proliferation by colony formation（C）and Edu assays（D，×200）. E：Wound healing assay to de⁃
tect the effect of TCEAL7 on cell migration（×40）. F：Transwell assay to examine the effect of TCEAL7 on cell migration and invasion（×100）. G，H：
Spheroid colony formation assay（G，×100）and flow cytometry analysis（H）in HGC⁃27 cells to explore the effect of TCEAL7 on the stem characteristics
**
*
of GC. P < 0.05，P < 0.01 and *** P < 0.001.
图9 TCEAL7在GC中的表达及功能
Figure 9 The expression and functions of TCEAL7 in GC