Page 26 - 南京医科大学自然版

第44卷第8期
·1054 · 南 京 医 科 大 学 学 报 2024年8月

A 2.0 * B 2.0 C 1.5 ** D 2.0 *
Relative proliferation rate 1.5 Relative proliferation rate 1.5 Relative proliferation rate 1.0 Relative proliferation rate 1.5

1.0
1.0
1.0
0.5
0.5
0.5
0.5
0
Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00%
CPO⁃PCL CPO⁃PCL CPO⁃PCL CPO⁃PCL
A：Hypoxia and osteogenic differentiation culture（Day 7）. B：Hypoxia and normal culture（Day 7）. C：Normoxia and osteogenic differentiation cul⁃
*
**
ture（Day 7）. D：Normoxia and normal culture（Day 7）. Compared with the control group，P < 0.05 and P < 0.01（n=3）.
图1 培养7 d MTT检测不同浓度CPO⁃PCL处理的ADMSC增殖情况
Figure 1 The proliferation of ADMSC after treatment with different concentrations of CPO⁃PCL for 7 days detected by
MTT

A 1.5 B 1.5 C 1.5 D 2.0
Relative proliferation rate 1.0 Relative proliferation rate 1.0 Relative proliferation rate 1.0 Relative proliferation rate 1.5

1.0
0.5
0.5
0.5
0.5
0
Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00% 0 Control 0.10% 0.25% 0.50% 1.00%
CPO⁃PCL CPO⁃PCL CPO⁃PCL CPO⁃PCL
A：Hypoxia and osteogenic differentiation culture（Day 14）. B：Hypoxia and normal culture（Day 14）. C：Normoxia and osteogenic differentiation
culture（Day 14）. D：Normoxia and normal culture（Day 14）（n=3）.
图2 培养14 d MTT检测不同浓度CPO⁃PCL处理的ADMSC增殖情况
Figure 2 The proliferation of ADMSC after treatment with different concentrations of CPO⁃PCL for 14 days detected by
MTT

A 6 *** *** B 4 *** C 2.0 *** D 1.5
Relative area of ALP 4 2 *** *** Relative area of ALP 3 2 1 * Relative area of ALP 1.5 Relative area of ALP 1.0

1.0
0.5
0.5
0 0 0 0
Control 0.10% 0.25% 0.50% 1.00% Control 0.10% 0.25% 0.50% 1.00% Control 0.10% 0.25% 0.50% 1.00% Control 0.10% 0.25% 0.50% 1.00%

CPO⁃PCL CPO⁃PCL CPO⁃PCL CPO⁃PCL
A：Hypoxia and osteogenic differentiation culture（Day 7）. B：Hypoxia and normal culture（Day 7）. C：Normoxia and osteogenic differentiation cul⁃
*
ture（Day 7）. D：Normoxia and normal culture（Day 7）. Compared with the control group，P < 0.05 and *** P < 0.001（n=3）.
图3 ALP检测缺氧或常氧培养7 d不同浓度CPO⁃PCL对ADMSC成骨分化培养和正常培养的影响
Figure 3 The effects of different concentrations of CPO⁃PCL on ADMSC with osteogenic differentiation culture or normal
culture under hypoxia or normoxia for 7 days detected by ALP

1.00%浓度的 CPO⁃PCL 微粒对 ADMSC 成骨诱导明 促进细胞成骨分化，ALP相对含量增多，而常氧正常
显，ALP 相对含量增多（P < 0.05），缺氧正常培养 培养14 d，未见ALP相对含量增多（图4）。
14 d，1.00% CPO⁃PCL微粒显著促进ADMSC向成骨 综上所述，缺氧成骨分化培养条件下，0.10%~
方向分化，ALP相对含量增多（P < 0.001）；常氧成骨 1.00% CPO⁃PCL微粒均可促进ADMSC成骨分化；缺
分化培养14 d，0.50%和1.00%的CPO⁃PCL微粒显著 氧正常培养或者常氧成骨分化培养条件下，1.00%