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A 10 HepG2 B HepG2
sh⁃EGFP 400 *
8 sh⁃ME3
nm） 6 * sh⁃EGFP 300
（450 D 4 Number of colony 200
2
sh⁃ME3 100
0
0 1 2 3 4
Time（d） 0
sh⁃EGFP sh⁃ME3
sh⁃EGFP sh⁃ME3
C
500
sh⁃EGFP
sh⁃ME3
Migration （per field） 400 * *
300
Number of cells 200
Invasion 100
0
Migration Invasion
A：CCK⁃8 assay showed that treatment with sh⁃ME3 decreased the cell viability of HepG2 cells at 1，2，3 and 4 days（n=3）. B：ME3 knockdown
inhibited cell clone formation in HepG2 cells（n=3）. C：Transwell and invasion assays indicated that ME3 knockdown inhibited the migration and
*
invasion abilities of HepG2 cells（×40，n=3）. P < 0.05.
图3 敲低ME3可以抑制HCC细胞的增殖、迁移和侵袭
Figure 3 ME3 knockdown inhibited the proliferation，migration and invasion of HCC cells
A 15 B
Vector 600 *
Flag⁃ME3
nm） 10 * Vector
（450 D 5 Number of colony 400
0 200
0 1 2 3 4 Flag⁃ME3
Time（d） 0
Vector Flag⁃ME3
Vector Flag⁃ME3
C
300
Vector
Flag⁃ME3
Migration （per field） 200 * *
Invasion Number of cells 100
0
Migration Invasion
A：CCK⁃8 assay showed that treatment with Flag⁃ME3 increased the cell viability of SMMC⁃7721 cells at 1，2，3 and 4 days（n=3）. B：ME3
over expression promoted cell clone formation in SMMC⁃7721 cells（n=3）. C：Transwell and invasion assays indicated that ME3 over expression promoted
*
the migration and invasion abilities of SMMC⁃7721 cells（×40，n=3）. P < 0.05.
图4 过表达ME3可以促进HCC细胞的增殖、迁移和侵袭
Figure 4 ME3 overexpression promoted the proliferation，migration，and invasion of HCC cells
