Page 28 - 南京医科大学自然版

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·1642 · 南 京 医 科 大 学 学 报 2024年12月

A B C P=0.004
（%） 100 High expression of
4 538 lncRNAs dominantly expressed in testis 10 P=0.003 LINC01424
normalized LINC01424 expression Survival probability 40
phyloP>0 and phastCons>0.3 8 80 Low expression of
60
74 CT⁃lncRNAs 6 LINC01424
Gene differential expression analysis 4 20
19 CT⁃lncRNAs 2 0
Survival analysis 0 10 20 30 40 50 60
TCGA tumor GTEx normal Survival time（months）
LINC01424
D E

50
10
40 8
TPM 30 6
20 Relative LINC01424 level in murine tissues
10 4
0 n= 663541258432240663 21 152176246215241255209197202246205159139459504174 9 10 373406375555515 9 429432 85 4 226578162803619180328283245604701187241359361653142156755 2 0
Lung
Uterus
Vagina
Liver
Brain⁃substantia nigra
Brain⁃hypothalamus
Ovary
Esophagus⁃muscularis
Esophagus⁃mucosa
Esophagus⁃gastroesophageal junction
Kidney⁃cortex
Cells⁃cultured fibroblasts
Skin⁃sun exposed
Pituitary
Brain⁃anterior cingulate cortex
Prostate
Testis
Adipose⁃subcutaneous Artery⁃aorta Artery⁃tibial Bladder Brain⁃caudate Brain⁃frontal cortex Cells⁃EBV⁃transformed lymphocytes Colon⁃sigmoid Heart⁃atrial appendage Kidney⁃medulla Muscle⁃skeletal Small intestine⁃terminal lleum Stomach Thyroid Whole blood Tracgea Heart Spinal cord Colon Liver Thyroid Kindey Spleen Stomach Eye Brian Lung Testis
Spleen
Pancreas
Brain⁃cortex
Brain⁃hippocampus
Brain⁃cerebellar hemisphere
Brain⁃amygdala
Heart⁃left ventricle
Skin⁃not sun exposed
Minor⁃salivary gland
Nerve⁃tibial
Brain⁃spinal cord
Artery⁃coronary
Brain⁃putamen
Brain⁃cerebellum
Brain⁃nucleus accumbens
Cervix⁃ectocervix
Colon⁃transverse
Fallopian⁃tube
Cervix⁃endocervix
Adrenal gland
Adipose⁃visceral
Breast⁃mammary tissue
F
M 1 M 2 M 3
G
5 000 bp-
5 000 bp- 3 000 bp-
2 000 bp- 2 000 bp-
1 500 bp-
I Nuclear
Cytoplasmic
H DAPI LINC01424 Merge
GAPDH
MALAT1
LINC01424
0 20 40 60 80 100
RNA（%）
A：Flowchart of the study. B：LINC01424 expression in ESCC and normal tissues in TCGA and GTEx databases（n=82）. C：Analysis of the correla⁃
tion between LINC01424 expression and the prognosis of ESCC patients by TCGA database. D：Expression of LINC01424 in a variety of normal human
tissues in GTEx database. E：Expression of LINC01424 in multiple normal tissues of mice（n=3）. F：Conservativeness analysis of LINC01424 in UCSC
Xena database. G：RACE assay identified the full⁃length sequence of LINC01424 in ESCC cells（n=3）. M：DNA marker；1：5′RACE of LINC01424；2：3′
RACE of LINC01424；3：the full⁃length of LINC01424. H，I：The distribution of LINC01424 in ECA109 cells detected by RNA⁃FISH（H，×400）and
qRT⁃PCR（I，n=3）.
图1 ESCC中CT⁃LINC01424的筛选
Figure 1 Screening of CT⁃LINC01424 in ESCC