Page 31 - 南京医科大学自然版

第44卷第12期 曹 靖，肖志寒，王子辰，等. 超保守癌/睾丸⁃LINC01424表达影响食管鳞癌的进展［J］.
2024年12月 南京医科大学学报（自然科学版），2024，44（12）：1638-1648 ·1645 ·

A B 1.0 C
● Control
1.0 ***
KD
■
（cm 3 ） 0.8 ● ● *** （g） 0.8
Tumor volume 0.4 ● ● ■ ■ ■ Tumor weight 0.4
Control 0.6 ● ■ 0.6
KD ■
0.2
0.2
0 0
8 12 15 18 21 Control KD
Time（d）
D E
1.5 100
***
level *** Control KD （%） 80
LINC01424 1.0 Ki⁃67 positive cells 60

40
Relative 0.5 20

0 0
Control KD Control KD
A：Tumor formation in nude mice in LINC01424⁃knockdown and control groups. B：Tumor growth curves of xenografts in LINC01424⁃knockdown
and control groups. C：The tumor weight was measured at the end point. D：qRT⁃PCR assay to verify of the expression of LINC01424 in tumors. E：Ki⁃67
***
staining in LINC01424⁃knockdown and control tumor tissues（×200）. KD：LINC01424⁃knockdown； P < 0.001（n=5）.
图4 敲降CT⁃LINC01424可以抑制ESCC的生长
Figure 4 Knockdown of CT⁃LINC01424 inhibited the growth of ESCC

A
1a st
1
Rank >3
0
sd

LINC01424 Score
NR2C1 7.9e-23
PAXIP1⁃AS1 3.11e-22
INTS6⁃AS1 3.06e-21
DNAJC9⁃AS1 1.24e-20
ENO1⁃AS1 1.35e-20
SNORD94 2.09e-20
METTL3 2.2e-20
RGS5 2.25e-20
MELTF⁃AS1 7.62e-20
KNTC1 8.09e-20
B C P < 0.001 D P < 0.001
GSE53624 8 9
9 r=0.296，P=0.001
expression 8 expression 6 expression 8
Normalized KNTC1 7 6 Normalized LINC01424 4 Normalized KNTC1 7 6

5 2 5
2 4 6 8 Normal tissue Tumor tissue Normal tissue Tumor tissue
Normalized LINC01424 expression （n=119） （n=119） （n=119） （n=119）
A：TOP 10 genes associated with LINC01424 expression in MEM database. B：Correlation of LINC01424 with KNTC1 expression in GSE53624
database. C，D：Expression of LINC01424（C）and KNTC1（D）in ESCC tissue and pericarcinomatous normal tissue.
图5 LINC01424靶基因预测
Figure 5 Prediction of target gene for LINC01424