Page 39 - 南京医科大学自然版

第44卷第12期 季明德，廖俊进，高彩月，等. SAMD13通过激活ERK1/2对胶质瘤细胞增殖和侵袭的影响［J］.
2024年12月 南京医科大学学报（自然科学版），2024，44（12）：1649-1656 ·1653 ·

A 4 ** B 1.5
shSAMD13⁃2
pIRES2⁃SAMD13
pIRES2⁃EGFP protein levels 3 shCTR shSAMD13⁃1 shSAMD13⁃3 protein levels ** *

SAMD13 14 kDa 2 SAMD13 14 kDa 1.0
Relative SAMD13 1 Relative SAMD13
β⁃actin 42 kDa β⁃actin 42 kDa 0.5

0
shSAMD13⁃2
pIRES2⁃EGFP 0.0 shSAMD13⁃1 shSAMD13⁃3
shCTR
pIRES2⁃SAMD13
A：U373 cells were transfected with pIRES2⁃EGFP and pIRES2⁃SAMD13 plasmids for 48 h，and then SAMD13 expression was examined by West⁃
ern blot. B：U373 cells were transfected with shCTR，shSAMD13⁃1，shSAMD13⁃2，and shSAMD13⁃3 plasmids for 48 h，and then SAMD13 expression
*
**
was detected by Western blot. P < 0.05 and P < 0.01（n=3）.
图2 SAMD13过表达和小干扰质粒对U373细胞中SAMD13表达的影响
Figure 2 Effects of SAMD13 overexpression and shRNA plasmids on SAMD13 expression in U373 cells

A B C
5 pIRES2⁃EGFP pIRES2⁃SAMD13 100 **
** 80
Relative OD value 3 2 Relative cell area/ （%） total area 60
4
40

0 1 20 0
pIRES2⁃SAMD13
pIRES2⁃SAMD13
pIRES2⁃EGFP pIRES2⁃EGFP

U373 cells were transfected with pIRES2⁃EGFP and pIRES2⁃SAMD13 plasmids. A：Cell proliferation was detected by CCK⁃8. B：Cell invasion was
**
observed by Transwell experiment. Scale bar=200 μm. P < 0.01（n=3）.
图3 过表达SAMD13对U373细胞增殖和侵袭的影响
Figure 3 Effects of SAMD13 overexpression on U373 cell proliferation and invasion

A B C
1.5 shCTR shSAMD13 20
**
Relative OD value 1.0 Relative cell area/ （%） total area 10 5
**
15
0.5

0.0 0
shCTR shCTR
shSAMD13⁃1
shSAMD13⁃1
U373 cells were transfected with shCTR and shSAMD13 plasmids. A：Cell proliferation was detected by CCK⁃8. B：Cell invasion was observed by
**
Transwell experiment. Scale bar=200 μm. P < 0.01（n=3）.
图4 沉默SAMD13对U373细胞增殖和侵袭的影响
Figure 4 Effects of SAMD13 silencing on U373 cell proliferation and invasion

和侵袭的机制，在 U373 细胞中分别转染 pIRES2⁃ Akt1和STAT3的磷酸化无明显影响（图5A）。此外，
EGFP 和 pIRES2⁃SAMD13 质粒，检查了一些信号分 在U373细胞中转染shCTR 和shSAMD13质粒，结果
子或转录因子（Akt1、ERK1/2和STAT3）的表达和磷 表明，通过转染shSAMD13质粒沉默SAMD13后，可
酸化修饰，发现通过转染pIRES2⁃SAMD13质粒过表 显著减弱 ERK1/2 的磷酸化，而对 Akt1 和 STAT3 的
达 SAMD13 后，可显著增强 ERK1/2 的磷酸化，而对 磷酸化也无明显影响（图5B）。