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第45卷第11期 林书慧，钱萌森，朱 静，等. METTL3介导的KIF11 mRNA m6A修饰通过PI3K/AKT信号通路促进结
2025年11月 直肠癌进展［J］. 南京医科大学学报（自然科学版），2025，45（11）：1546-1562 ·1557 ·
A B
FMC1 2 Volcano plot
CENPQ
KLHL5 40
SYNE2
ITGA9
TOP2A 1
CCNG1
CD164
DYNLT3 30
ERLIN2
DDAH1 0 Signficant
SCN4A （FDR）
ATP2B4
FAM117B 20 Up（n=114）
SLC16A9
IDI1 Down（n=52）
PROM1 -1 -lg
EAPP
FOXC1 10 Normal（n=13 262）
RBFOX2
TMEM170B
TTC31
CD9 -2
CDK19 0
HMCN1
SLC16A7
FAT4 -5 0 5
SESN3
ARHGAP42
UHMK1 log2|FC|
P3H2
KIF11
NUSAP1
BTBD8
CXADR
MAN2A1
PIK3R1 C
CLDN2 PI3K⁃Akt signaling pathway P value P.adjust
SPARC
TMEM132B
DYNC2H1 0.1
MSTRG.18981 0.004 5 0.040 5
ENSG00000188223
GLDN 0
ST6GALNAC5
MSTRG.11779 enrichment score -0.1
MSTRG.18069 Running
MAML2
PEG10 -0.2
ENSG00000285897
GPRASP3 -0.3
MSTRG.11048
ADORA2A
ENSG00000282988
SRCAP
ENSG00000286070
KRT13
CASS4 5
HYPK
SYS1⁃DBNDD2
MSTRG.14704 Ranked list metric 0
ABCA12
MSTRG.12453 -5
DDIT4
H2AJ
UPP1 5 000 10 000
CHAC1
RUNDC3A
MON1A Rank in ordered dataset
ARTN
PER1
ZC3H12A
EGLN2
MSTRG.13937
PHLDA3 D
GDF15
JUN HCT116 DLD1 SW480
H2AC18
H2AC19
H2AC25
HIC2
TRIB3
DUSP8
GADD45B shKIF11⁃1 shKIF11⁃2 shKIF11⁃1 shKIF11⁃2
EHD1 oe⁃KIF11
RRAD oe⁃NC
LIN28A shNC shNC
MSTRG.15271
SLC25A45
AGAP6
MSTRG.21447 p⁃AKT 60 kDa
COL17A1
MRM1
TMEM198
H2BC4 AKT 60 kDa
PER2
PLK3
DOT1L
MSTRG.12612
BTG2 p⁃PI3K 100 kDa
MSTRG.15887
H2BC12
MSTRG.2295
CDKN1A PI3K 85 kDa
H3C10
DUSP1
TP53I3
MSTRG.181 GAPDH 36 kDa
MSTRG.4818
MSTRG.4860
CPA4
KRT15
FOSL1
KRTAP1⁃3
CCDC62
SESN1
ARRDC4 E
MSTRG.16143 HCT116 DLD1
MSTRG.74
DDIT3
SYP
H1⁃2
ATF3
DYNLT2
ARRDC2
SERPINE1
BHLHE41 shMETTL3+oe⁃KIF11 shMETTL3+oe⁃KIF11
GPR87 shMETTL3+oe⁃NC shMETTL3+oe⁃NC
MSTRG.7894
INPP5D
H2BC21
SESN2 shNC shNC
FSD2
CXCL3
FOSB
RGS16 p⁃AKT
KLF11 60 kDa
PLK2
H4C15
H2BC5
TNFRSF10C AKT 60 kDa
H2AC6
KRT80
IL12A
THAP2 p⁃PI3K 100 kDa
NECTIN4
AKR1B10
PLA2G4C
KHDC4 PI3K 85 kDa
MSTRG.3934
MSTRG.180
MSTRG.18390
MSTRG.6453 GAPDH 36 kDa
MSTRG.10325
PYGM
H2BC26
KLHL41
H2BC11
PRDM1
TNFAIP3
GRHL2
MSTRG.4706
H2BC15
RSRP1
H2BC6
MSTRG.9969
NC1 NC2 NC3 S11 S12 S13
A：Heatmap of the results of cellular RNA⁃seq analysis. B：Volcano plot of the results of cellular RNA⁃seq analysis. C：GSEA enrichment analysis
showed that KIF11 was associated with PI3K/AKT signaling pathway. D：The expression level of related proteins in the PI3K/AKT signaling pathway
after knockdown or overexpression of KIF11 was detected by WB. E：WB was performed to detect the effect of the METTL3/KIF11 axis on the expression
level of related proteins in the PI3K/AKT signaling pathway（n=3）.
图8 KIF11通过PI3K/AKT信号通路促进CRC进展
Figure 8 KIF11 promoted CRC progression through the PI3K/AKT signaling pathway
