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A B
HCT116 shNC DLD1 shNC
2.0 2.0
shMETTL3+oe⁃NC shMETTL3+oe⁃NC
nm） 1.5 shMETTL3+oe⁃KIF11 nm） 1.5 shMETTL3+oe⁃KIF11
（450 D 1.0 *** *** （450 D 1.0 *** ***
0.5
0.5
0 0
0 1 2 3 4 0 1 2 3 4
Time（d） Time（d）

C D
shNC shMETTL3+oe⁃NC shMETTL3+oe⁃KIF11 shNC
shMETTL3+oe⁃NC
shMETTL3+oe⁃KIF11
2 500
*
HCT116 Number of cells 1 500 * * *
2 000
1 000
500
0
HCT116 DLD1
DLD1

E HCT116 DLD1
Hoechst EdU Merge Hoechst EdU Merge

shNC

shMETTL3+ oe⁃NC

shMETTL3+ oe⁃KIF11 500 μm

F G H
shNC shNC shMETTL3+oe⁃NC shMETTL3+oe⁃KIF11 shNC
shMETTL3+oe⁃NC shMETTL3+oe⁃NC
shMETTL3+oe⁃KIF11 1 200 shMETTL3+oe⁃KIF11
（%） 60 ** ** * ** HCT116 800 * ** **
Edu positive cells 20 Number of cells 400 **
40

0
HCT116 DLD1 DLD1 0 HCT116 DLD1

A，B：CCK⁃8 assays showed the effects of METTL3 and KIF11 on HCT116（A）and DLD1（B）cell proliferation ability. C，D：Clond formation assays
showed effects of METTL3 and KIF11 on HCT116（A）and DLD1（B）cell proliferation ability. E，F：EdU assays showed effects of METTL3 and KIF11
on HCT116（A）and DLD1（B）cell proliferation ability.（×100，scale bar=500 μm）. G，H：The effects of METTL3 and KIF11 on cell migration ability
**
*
were measured by transwell assay（×100，scale bar=100 μm）. P < 0.05，P < 0.01，and *** P < 0.001（n=3）.
图7 METTL3通过调控KIF11促进CRC增殖和迁移
Figure 7 METTL3 promoted CRC proliferation and migration by regulating KIF11