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A shNC B HCT116 DLD1 C 5 D SW480
1.5 shKIF11⁃1 * 4 ** oe⁃KIF11
**
Relative PROM1 mRNA expression 1.0 PROM1 shNC shKIF11⁃1 shNC shKIF11⁃1 115 kDa Relative PROM1 mRNA expression 3 2 1 PROM1 oe⁃NC 115 kDa

0.5
42 kDa
ACTIN
0 ACTIN 42 kDa 0 oe⁃NC oe⁃KIF11
HCT116 DLD1 SW480
E F
HCT116 shNC DLD1 shNC
2.0 2.0
shKIF11⁃1+oe⁃NC shKIF11⁃1+oe⁃NC
nm） 1.5 shKIF11⁃1+oe⁃PROM1 nm） 1.5 shKIF11⁃1+oe⁃PROM1
（450 D 1.0 *** *** （450 D 1.0 *** ***
0.5
0.5
0 0
0 1 2 3 4 0 1 2 3 4
G H J
shNC shKIF11⁃1+oe⁃NC shKIF11⁃1+oe⁃PROM1 shNC shNC
shKIF11⁃1+oe⁃NC shKIF11⁃1+oe⁃NC
shKIF11⁃1+oe⁃PROM1 shKIF11⁃1+oe⁃PROM1
2 000 （%） 60 *** **
HCT116 Number of cells 1 500 ** ** * * EdU positive cells 40 * **
1 000
500
0 20 0
HCT116 DLD1 HCT116 DLD1
DLD1

I HCT116 DLD1
Hoechst EdU Merge Hoechst EdU Merge

shNC

shKIF11⁃1+ oe⁃NC

shKIF11⁃1+ oe⁃PROM1

500 μm
A，B：The expression level of PROM1 after knockdown of KIF11 was detected by qRT⁃PCR（A）and WB（B）. C，D：The expression level of PROM1
after overexpression of KIF11 was detected by qRT⁃PCR（C）and WB（D）. E，F：CCK⁃8 assays showed that overexpression of PROM1 partially rescued
the effect of KIF11 knockdown on HCT116 and DLD1 cell proliferation. G，H：Clone formation assays showed that overexpression of PROM1 partially
rescued the effect of KIF11 knockdown on HCT116 and DLD1 cell proliferation. I，J：EdU assays showed that overexpression of PROM1 partially res⁃
*
**
cued the effect of KIF11 knockdown on HCT116 and DLD1 cell proliferation.（×100，scale bar=500 μm）. P < 0.05，P < 0.01，and *** P < 0.001（n=3）.
图9 KIF11通过调控PROM1促进CRC增殖
Figure 9 KIF11 promoted CRC proliferation by regulating PROM1

chemotherapy tolerance in CRC through the JAK2/ gression of hepatocellular carcinoma via the OCT4
［32］ ［33］
STAT3 signaling pathway ；KIF11 promotes the pro⁃ pathway . Our study demonstrated that KIF11 was