Page 21 - 南京医科大学自然版

第45卷第11期 林书慧，钱萌森，朱 静，等. METTL3介导的KIF11 mRNA m6A修饰通过PI3K/AKT信号通路促进结
2025年11月 直肠癌进展［J］. 南京医科大学学报（自然科学版），2025，45（11）：1546-1562 ·1551 ·

A HCT116 B DLD1 C SW480
3 3 2.5
shNC shNC *** oe⁃NC
nm） 2 shKIF11⁃1 *** nm） 2 shKIF11⁃1 ** nm） 2.0 oe⁃KIF11 ***
shKIF11⁃2
shKIF11⁃2
***
（450 D 1 （450 D 1 （450 1.5
1.0
D
0.5
0 0 0
0 1 2 3 4 0 1 2 3 4 0 1 2 3 4
Time（d） Time（d） Time（d）
D shNC shKIF11⁃1 shKIF11⁃2 E shNC F SW480 G 2 000 **
shKIF11⁃1
1 500 shKIF11⁃2 1 500
Number of colonies 500 * ** ** ** 500
HCT116 1 000 oe⁃NC Number of colonies 1 000

0 0 oe⁃NC oe⁃KIF11
DLD1 HCT116 DLD1 oe⁃KIF11 SW480

H I
HCT116 DLD1
Hoechst EdU Merge Hoechst EdU Merge ** * shNC
shNC （%） 50 ** ** shKIF11⁃1
40
shKIF11⁃2
30
shKIF11⁃1 Edu positive cells 20
10
shKIF11⁃2 500 μm 0 HCT116 DLD1

J SW480 K 0.5 *
Hoechst EdU Merge （%） 0.4
oe⁃NC Edu positive cells 0.3
0.2
oe⁃KIF11 500 μm 0.1 0

oe⁃NC oe⁃KIF11
SW480
L M shNC N O
shNC shKIF11⁃1 shKIF11⁃2 shKIF11⁃1 SW480 800 *
shKIF11⁃2 ** 600
HCT116 800 ** ** ** oe⁃NC Number of cells 400
1 200

DLD1 Number of cells 400 oe⁃KIF11 200 0 oe⁃NC oe⁃KIF11

0 SW480
HCT116 DLD1
A，B：The effect of KIF11 knockdown on cell proliferation was evaluated by CCK⁃8 assay in HCT116（A）and DLD1（B）cells. C：The effect of
KIF11 overexpression on SW480（C）cell proliferation was assessed using CCK⁃8 assay. D，E：Colony formation assays were conducted to analyze the im⁃
pact of KIF11 knockdown on HCT116 and DLD1 cell proliferation（×100，scale bar=100 μm）. F，G：The effect of KIF11 overexpression on SW480 cell
proliferation was tested via colony formation assays. H，I：EdU assays were performed to examine the effect of KIF11 knockdown on HCT116 and DLD1
cell proliferation（×100，scale bar=500 μm）. J，K：EdU assays were used to evaluate the effect of KIF11 overexpression on SW480 cell proliferation
（×100，scale bar=500 μm）. L，M：Transwell migration assays were conducted to determine the effect of KIF11 knockdown on the migration ability of
HCT116 and DLD1 cells（×100，scale bar=100 μm）. N，O：The impact of KIF11 overexpression on the migration ability of SW480 cells was assessed by
**
*
transwell assays（×100，scale bar=100 μm）. Compared with the shNc group，P < 0.05，P < 0.01，and *** P < 0.001（n=3）.
图2 KIF11促进结直肠癌进展
Figure 2 KIF11 promoted colorectal cancer cell progression