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A B
5 COAD READ
Normal tissues n=275 n=349 n=92 *
4
（TPM+1） 3 （TPM+1） 5 4 n=318
*
Tumor tissues
lg 2
1 3

0 KIF11 expression⁃log 2 2
BLCA BRCA CESC CHOL COAD ESCA GBM HNSC KICH KIRC KIRP LIHC LUAD LUSC PAAD PCPG PRAD READ STAD THCA UCEC 1
TCGA 0
Normal tissues
Tumar tissues Tumar tissues
Normal tissues
C 0 D 200
** 150
（ΔCT） -5 100
20
expression -10 Relative KIF11 expression （normalized to normal tissues） 15
10
KIF11 -15 -5 5 0

-20 -10
Normal tissues Tumor tissues Low expression High expression
（n=30） （n=30） （n=8） （n=22）

A：Expression of KIF11 in TCGA in various types of cancers. B：GEPIA database showed that KIF11 was highly expressed in CRC tissues（COAD：
colon adenocarcinoma；READ：rectum adenocarcinoma）. C，D：KIF11 expression levels in 30 pairs of clinical tissues were examined（C）and divided into
**
*
high and low expression groups by qRT⁃PCR（D）. P <0.05 and P < 0.01.
图1 KIF11在结直肠癌组织中高表达
Figure 1 KIF11 was highly expressed in CRC tissues

表2 CRC患者KIF11的表达水平与临床病理参数的相关性 pression of KIF11 enhanced cell viability（Figure 2C，
Table 2 Correlations between KIF11 expression and clini⁃ F，G）. In addition，the EdU assay revealed the same re⁃
copathological characteristics in CRC patients sults（Figure 2H- K）. According to transwell assay，
［n（%）］
KIF11 knockdown weakened the migration ability of
Expression of KIF11
Variable P cells compared with the control，while overexpression
Low（n=8） High（n=22）
Age（years） >0.990 of KIF11 significantly enhanced the cell migration
≤60 3（37.5） 07（31.8） capacity（ Figure 2L- O ）. Flow cytometry analysis
>60 5（62.5） 15（68.2） revealed that KIF11 knockdown significantly increased
Sex >0.990 apoptosis rate in CRC cells，whereas KIF11 overexpres⁃
Male 5（62.5） 12（54.5）
sion suppressed cell apoptosis（Figure 3）.
Female 3（37.5） 10（45.5）
2.3 KIF11 promoted CRC progression in vivo
TNM stage 0.078
To explore whether KIF11 promotes the progres⁃
Ⅰ/Ⅱ 5（62.5） 05（22.7）
Ⅲ/Ⅳ 3（37.5） 17（77.3） sion of CRC in vivo，we conducted subcutaneous xeno⁃
Lymph node metastasis 0.034 graft experiments. HCT116 cells transfected with shNC
Negative 6（75.0） 06（27.3） or shKIF11⁃1 were inoculated into mice（five mice per
Positive 2（25.0） 16（72.7） group），and the tumors were compared after 21 days of
Distant metastasis 0.049
injection（Figure 4A）. The results showed that com⁃
Negative 6（75.0） 07（31.8）
pared with the control group，the subcutaneous tumors
Positive 2（25.0） 15（68.2）
with KIF11 knockdown exhibited lower volume and
the knockdown of KIF11 inhibited the proliferative weight（Figure 4B，C）.
ability of cells（Figure 2A，B，D，E），while overex⁃ 2.4 METTL3⁃regulated m6A modification enhanced