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A B C
pIRES2⁃EGFP + + - 1.5 pIRES2⁃EGFP + + -
pIRES2⁃FBXO6 - - + pIRES2⁃FBXO6 - - +
DMSO + - - 1.0 DMSO + - -
Perifosine - + + mRNA levels Perifosine - + +
FBXO6 487 bp Relative FBXO6 0.5 ** FBXO6 33 kDa
GAPDH 203 bp β⁃actin 42 kDa
0
pIRES2⁃EGFP + + -
pIRES2⁃FBXO6 - - +
DMSO + - -
Perifosine - + +
D E
1.5 1.5
Relative FBXO6 protein levels 1.0 ** Relative cell proliferation rate 1.0 **

0.5
0.5
0 0
pIRES2⁃EGFP + + - pIRES2⁃EGFP + + -
pIRES2⁃FBXO6 - - + pIRES2⁃FBXO6 - - +
DMSO + - - DMSO + - -
Perifosine - + + Perifosine - + +
60
F G
pIRES2⁃EGFP + + -
Relative cell area/
pIRES2⁃FBXO6 - - + （%） 40
DMSO + - -
Perifosine - + + total area 20 **

0
×100 pIRES2⁃EGFP + + -
pIRES2⁃FBXO6 - - +
DMSO + - -
Perifosine - + +
U87 cells were transfected with pIRES2⁃EGFP or pIRES2⁃FBXO6 followed by Perifosine incubation. The mRNA and protein levels of FBXO6 were
detected by RT⁃PCR（A：electrophoretic bands of RT⁃PCR；B：statistical chart）and Western blot（C：electrophoretic bands of Western blot；D：statistical
chart）. The cell proliferation and invasion levels were detected by CCK⁃8（E）and Transwell（F：representative images，scale bar=200 μm；G：statistical
**
chart）. Compared with the DMSO + pIRES2⁃EGFP group，P < 0.01（n=3）.
图6 FBXO6过表达对Perifosine抑制U87细胞增殖和侵袭作用的影响
Figure 6 The effects of FBXO6 overexpression on the proliferation and invasion of U87 cells in response to Perifosine

Conflict of Interests： guidance and data analysis.WANG Yingwei was responsible for
The author’s affiliation and the publishing department of project guidance and paper revision. QIU Wen and ZHAO Chen⁃
this journal are affiliated with Nanjing Medical University，but hui were responsible for research design，data analysis，and pa⁃
there is no conflict of interest in this work. per revision.
作者贡献声明：
［参考文献］
高彩月负责生信分析、主体实验、数据分析、论文撰写，
曹丹丹和倪思琦负责部分实验和数据分析；张婧负责实验指 ［1］ WANG L M，ENGLANDER Z K，MILLER M L，et al. Ma⁃
导、数据分析；王迎伟负责课题指导、论文修改；邱文、赵晨卉 lignant glioma［J］. Adv Exp Med Biol，2023，1405：1-30
负责研究设计、数据分析、论文修改。 ［2］ OSTROM Q T，PATIL N，CIOFFI G，et al. CBTRUS statis⁃
Author’s Contributions： tical report：primary brain and other central nervous system
GAO Caiyue was responsible for bioinformatics analysis， tumors diagnosed in the United States in 2013 ⁃ 2017［J］.
main experiments，data analysis，and paper writing. CAO Dan⁃ Neuro Oncol，2020，22（12 Suppl 2）：1-96
dan and NI Siqi were responsible for part of the experiments and ［3］ GE H L，YAN Z，LIU D M，et al. Synergetic reorganiza⁃
data analysis. ZHANG Jing was responsible for experimental tion of the contralateral structure and function in patients