Page 44 - 南京医科大学自然版

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A B 150
P < 0.01
Relative CNN2 protein expression 1.0 Relative migration cells

Hard Normal 2.5 P < 0.01 （%） 100 P < 0.001
2.0
CNN2 13 kDa 1.5

Tublin 55 kDa 0.5 50

0
Hard Normal
0
Hard Normal Hard⁃shCNN2
A：Western blot analysis showed that the protein expression of CNN2 in liver cancer cells cultured on rigid substrates was higher than that in
normal culture medium. B：Both rigid substrates and CNN2 promote migration（n=3）.
图4 ECM力学信号调控CNN2促进肝癌细胞迁移
Figure 4 ECM mechanical signals regulate CNN2 to promote liver cancer cell migration

A B
Huh⁃7
1.5 P < 0.01 Control shWnt11 1.5 P < 0.01 1.5 P < 0.01
mRNA levels 1.0 Wnt11 45 kDa protein expression 1.0 protein expression 1.0

Relative Wnt11 0.5 CNN2 13 kDa Relative Wnt11 0.5 Relative CNN2 0.5

0 GAPDH 35 kDa 0 0
Control shWnt11 Control shWnt11 Control shWnt11
P < 0.01
100 P < 0.01
C
（%） 80
Control shWnt11 shWnt11+shCNN2 P < 0.01
Control
Relative migration cells 60

40

20

0
Control shWnt11
Control
shWnt11+shCNN2
A：qPCR was used to verify the transfection efficiency（n=6）. B：Western blot was used to investigate changes in protein expression. C：Migration
assay showing that liver cancer cells with simultaneous silencing of Wnt11 and CNN2（shWnt11+shCNN2）have a significantly reduced number of cells
passing through the Transwell chamber compared to the Wnt11 interference group（shWnt11），Bar chart showing cell percentage（scale bar=100 μm，n=3）.
图5 Wnt/PCP信号通路影响CNN2表达促进肝癌细胞群体性迁移
Figure 5 The Wnt/PCP signaling pathway regulates CNN2 to influence collective migration of liver cancer cells

移机制之间的精细交互，并探索靶向 ECM⁃细胞互 大量研究表明，Wnt/PCP 信号通路在肿瘤的细
作的精准干预策略，为抗转移治疗提供新的思路与 胞群体性迁移中起重要作用，ECM与Wnt/PCP信号
靶点。 通路协同调控肿瘤细胞的迁移 ［19- 20］ 。CNN2 作为