Page 41 - 南京医科大学自然科学版第1期

第41卷第1期 南京医科大学学报（自然科学版）
2021年1月 Journal of Nanjing Medical University（Natural Sciences） · 35 ·

·基础研究·

苦参碱调控Wnt/β⁃catenin途径抑制宫颈癌细胞系Caski细胞侵

袭迁移

郑荣芳 ，刘 伟 ，张芸中 ，樊学芬 ，郭钰珍 1*
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兰州大学第二医院妇科，麻醉科，甘肃 兰州 730000
1 2
［摘 要］ 目的：基于Wnt/β⁃catenin途径探索苦参碱抑制人宫颈癌细胞系Caski细胞侵袭迁移的分子机制。方法：Caski细胞
用不同浓度苦参碱处理后，MTT法检测细胞增殖抑制情况；Transwell小室侵袭迁移实验检测细胞侵袭迁移情况；ELISA法检测
细胞基质金属蛋白酶 9（matrix metalloproteinase 9，MMP⁃9）和血管内皮生长因子（vascular endothelial growth factor，VEGF）的分
泌变化；实时荧光定量PCR检测细胞糖原合成酶激酶3β（glycogen synthase kinase 3β，GSK⁃3β）、Wnt2B蛋白的表达情况；免疫
蛋白印迹检测细胞β连环蛋白（β⁃catenin）、磷酸化β连环蛋白（p⁃β⁃catenin）含量。结果：苦参碱对Caski细胞的增殖具有抑制作
用（P＜0.05），且呈现时间、浓度依赖性；苦参碱抑制Caski细胞的侵袭迁移率（P＜0.05），对Caski细胞分泌MMP⁃9、VEGF的能
力具有显著抑制作用（P＜0.05）；苦参碱可显著降低Caski细胞Wnt2B mRNA的表达及β⁃catenin的蛋白含量，增加Caski细胞中
GSK⁃3β mRNA的表达和p⁃β⁃catenin的蛋白含量。结论：苦参碱通过促进Caski细胞Wnt/β⁃catenin通路中GSK⁃3β mRNA表达，
提高p⁃β⁃catenin蛋白含量，降低Wnt2B mRNA表达、β⁃catenin蛋白含量以及Caski细胞内MMP⁃9、VEGF的分泌，进而抑制Caski
细胞的侵袭迁移。
［关键词］ 苦参碱；Caski细胞；Wnt/β⁃catenin通路；侵袭；迁移
［中图分类号］ R737.33 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）01⁃035⁃06
doi：10.7655/NYDXBNS20210107

Effects of matrine on invasion and migration of cervical cancer cell line Caski by Wnt/β⁃
catenin pathway

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ZHENG Rongfang ，LIU Wei ，ZHANG Yunzhong ，FAN Xuefen ，GUO Yuzhen 1*
1 Department of Gynecology，Department of Anesthesiology，Lanzhou University Second Hospital，Lanzhou 730000，
2
China
［Abstract］ Objective：This study aims to explore the molecular mechanism of matrine in inhibiting the invasion and migration of
human cervical cancer cell line Caski cells based on the Wnt/β⁃catenin pathway. Methods：Caski cells were treated with different
concentration of matrine. Methyl thiazolyl tetrazolium（MTT）assay was used to detect cell proliferation inhibition；Transwell chamber
invasion and migration assay was used to detect cell invasion and migration；ELISA assay was used to detect cell matrix metalloprotein
9（MMP⁃9）and vascular endothelial growth factor（VEGF）secretion；Quantitative real⁃time PCR（qRT⁃PCR）was used to detect cell
glycogen synthase kinase3β（GSK⁃3β）and wingless/integrated 2B（Wnt2B）expression；Western blot was used to detect cell β⁃catenin，p
⁃β⁃catenin protein content. Results：Matrine had inhibitory effect on the proliferation of cervical cancer cells Caski cells（P < 0.05）and
showed a time and concentration dependence. Matrine inhibited the invasion and migration of Caski cells（P < 0.05），and it had a
significant effect on Caski cells. The ability to secrete MMP⁃9 and VEGF has a significant inhibitory effect（P < 0.05）；matrine can
significantly reduce Wnt2B mRNA expression and β⁃catenin protein content in Caski cells，and increase GSK⁃3β mRNA expression in
Caski cells and p ⁃ β ⁃ catenin protein content. Conclusion：Matrine promotes the expression of GSK ⁃ 3βmRNA in Wnt/β ⁃ catenin
pathway of Caski cells，increases the content of p⁃β⁃catenin protein，reduces the expression of Wnt2B mRNA，the content of β⁃catenin
protein and the secretion of MMP⁃9 and VEGF in Caski cells，thereby inhibiting the invasion and migration of Caski cells.
［Key words］ matrine；Caski cell；Wnt/β⁃catenin pathway；invasion；migration
［J Nanjing Med Univ，2021，41（01）：035⁃040］
［基金项目］ 甘肃省自然科学基金项目（17JR5RA242）；兰州大学第二医院“萃英科技创新”计划（CY2017⁃BJ18）
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通信作者（Corresponding author），E⁃mail：guoyz@lzu.edu.cn