Page 22 - 南京医科大学学报自然科学版
P. 22
南京医科大学学报(自然科学版) 第41卷第12期
·1728 · Journal of Nanjing Medical University(Natural Sciences) 2021年12月
·基础医学·
MicroRNA⁃190调控衰老相关的脂肪能量代谢稳态
彭丹丹 ,李 言 2*
1
1 东南大学生命科学与技术学院,江苏 南京 210096;江南大学食品学院,江苏 无锡 214122
2
[摘 要] 目的:探究微小RNA(microRNA,miR)⁃190在衰老相关的能量代谢紊乱中的作用和潜在分子机制。方法:利用实时
荧光定量PCR(real⁃time fluorescent quantitative ⁃polymerase chain reaction,RT⁃PCR),对衰老小鼠脂肪组织中miR⁃190的表达水
平进行检测;构建细胞衰老模型,利用RT⁃PCR检测脂肪细胞中miR⁃190的变化;利用RT⁃PCR检测转染miR⁃190的脂肪细胞中
产热基因的变化;用双荧光素酶报告基因实验验证miR⁃190的靶基因;利用antagomir注射抑制衰老小鼠脂肪中产生miR⁃190,
检测代谢表征。结果:衰老进程中,脂肪组织中miR⁃190表达升高;衰老细胞模型中,miR⁃190的表达同样上调;脂肪细胞中过
表达 miR⁃190 会导致产热基因的表达降低;产热关键基因 PRDM16 是 miR⁃190 的直接靶基因;抑制衰老小鼠中 miR⁃190 的产
生,可以有助于改善衰老小鼠的能量代谢稳态。结论:miR⁃190可能通过靶向PRDM16影响脂肪组织产热,继而影响能量代谢
稳态,加速衰老。
[关键词] miR⁃190;衰老;脂肪组织;产热;能量代谢;PRDM16
[中图分类号] R339.6 [文献标志码] A [文章编号] 1007⁃4368(2021)12⁃1728⁃07
doi:10.7655/NYDXBNS20211203
MicroRNA⁃190 regulates aging⁃related fat energy metabolism homeostasis
1
PENG Dandan ,LI Yan 2
2
1 School of Life Science and Technology,Southeast University,Nanjing 210096;School of Food Science and
Technology,Jiangnan University,Wuxi 214122,China
[Abstract] Objective:This study aims to explore the role and potential molecular mechanism of microRNA(miR)⁃190 in aging⁃
related energy metabolism disorders. Methods:Real⁃time fluorescent quantitative PCR(RT⁃PCR)was used to detect the expression
level of miR⁃190 in the adipose tissue of aging mice;a cellular senescence model was constructed and RT⁃PCR was used to detect the
changes of miR⁃190 in adipocytes;RT⁃PCR was used to detect the changes in thermogenesis genes in the adipocytes transfected with
miR⁃190;the target genes of miR⁃190 were verified by the dual luciferase reporter gene experiment;the metabolic homeostasis was
determined in aged mice injected with miR⁃190 antagomir. Results:During the aging process,the expression of miR⁃190 in adipose
tissue was elevated;in the senescent cell model,the expression of miR⁃190 was also up⁃regulated;overexpression of miR⁃190 in
adipocytes would lead to a decrease in the expression of thermogenesis genes;the key genes for thermogenesis PRDM16 is the direct
target gene of miR⁃190;inhibiting the level of miR⁃190 in aging mice can help improve the energy metabolism homeostasis of aged
mice. Conclusion:miR⁃190 may affect adipose tissue heat production by targeting PRDM16,which in turn affects energy metabolism
homeostasis and accelerates aging.
[Key words] miR⁃190;aging;adipose tissue;thermogenesis;energy metabolism;PRDM16
[J Nanjing Med Univ,2021,41(12):1728⁃1734]
衰老通常伴随机体发生不可逆转的生理功能 谢稳态失衡通常包括能量消耗减少、脂肪堆积以及胰
下降,这其中就包括能量代谢稳态。衰老相关的代 岛素敏感性的降低等 [1-2] 。衰老相关的代谢稳态失衡
与很多代谢性疾病有着十分密切的关系,包括2型糖
[基金项目] 国家自然科学基金(31900841,32071166) 尿病、心血管疾病以及神经退行性疾病等 [3-6] 。因此,
∗ 探究衰老相关的代谢稳态失衡发生发展过程中的
通信作者(Corresponding author),E⁃mail:103008528@seu.edu.cn