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第41卷第2期                           南京医科大学学报(自然科学版)
                  2021年2月                   Journal of Nanjing Medical University(Natural Sciences)     ·181 ·


               ·基础研究·

                低频耳针电刺激降低内脏敏感性的机制探讨



                徐万里 ,周 帅 ,周静珠 ,赵瑞瑞 ,孙鲁宁 ,王永庆 ,薛明新 ,陈                         欢 ,张朝晖     1*
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                南京医科大学第一附属医院针灸科,江苏                南京   210029;南京中医药大学第二临床医学院,江苏               南京    210029;南京医
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                科大学第一附属医院药学部,江苏 南京               210029
               [摘   要] 目的:观察低频耳电针在降低内脏高敏感模型大鼠内脏敏感性中的作用。方法:SD大鼠随机分为空白组、模型组、
                耳针1组、耳针2组,每组10只。除空白组外,其余大鼠通过母婴分离加醋酸灌肠建立内脏高敏感大鼠模型。耳针1组与耳针
                2组接受2 Hz电针刺激30 min/d,10 d。通过腹外斜肌肌电(EMG)测定直肠扩张下大鼠内脏疼痛阈值,远端结肠HE染色观察
                病理改变,实时荧光定量PCR检测大鼠下丘脑nesfatin⁃1,促皮质素释放因子(CRF)及其受体1,2(CRF1/2R)及结肠5⁃羟色胺受
                体 4(serotonin receptor 4,5⁃HT4R)的 mRNA 表达情况。结果:模型组腹外斜肌放电曲线下面积(AUC)较空白组显著增加(P<
                0.05);与模型组相比,耳针 1 组 AUC 显著减少(P<0.05)。与空白组比较,模型组下丘脑 CRF、nesfatin⁃1、CRF1R、CRF2R 的
                mRNA表达均显著升高(P<0.05),远端结肠5⁃HT4R的mRNA表达有下降的趋势(P=0.067)。耳针1组下丘脑CRF、nesfatin⁃1、
                CRF1R、CRF2R的mRNA较模型组显著下降(均P<0.05),且远端结肠5⁃HT4R的mRNA表达显著提高(P<0.05)。耳针2组下
                丘脑 CRF、CRF2R 的 mRNA 较模型组显著下降(均 P<0.05),但较模型组 nesfatin⁃1、CRF1R 无显著差异,远端结肠 5⁃HT4R 的
                mRNA表达上调不显著(P>0.05)。结论:低频耳电针可缓解内脏高敏感大鼠的内脏敏感性,可能与调节下丘脑CRF、CRF1R、
                CRF2R、nesfatin⁃1,以及远端结肠5⁃HT4R的mRNA表达有关。
               [关键词] 耳针;内脏高敏感;nesfatin⁃1;促皮质素释放因子;肠易激综合征
               [中图分类号] R245                     [文献标志码] A                       [文章编号] 1007⁃4368(2021)02⁃181⁃06
                doi:10.7655/NYDXBNS20210205



                Study on the mechanism of low⁃frequency auricular electric stimulation to reduce visceral
                hypersensitivity

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                XU Wanli ,ZHOU Shuai ,ZHOU Jingzhu ,ZHAO Ruirui ,SUN Luning ,WANG Yongqing ,XUE Mingxin ,CHEN
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                Huan ,ZHANG Zhaohui  1*
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                Department of Acupuncture,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029;the Second
                Clinical School of Medicine,Nanjing University of Traditional Chinese Medicine,Nanjing 210029;Department of
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                Pharmacy,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029,China
               [Abstract] Objective:This study aims to explore the role of‘Brain⁃gut’regulation in low⁃frequency auricular electro⁃acupuncture
                on maternal separation combined with enema induced visceral hypersensitivity rats. Methods:Visceral hypersensitivity was
                established by maternal separation combined with acetic acid enema in SD rats,which were randomly divided into the normal control
               (NC)group,model control(MC)group,auricular acupuncture group 1(AA1)and auricular acupuncture group 2(AA2). Rats in the
                AA1 group and AA2 group were received electroacupuncture stimulation(2 Hz,30 min/d)for consecutive 10 days. The visceral
                sensitivity of rats was evaluated by electromyographic(EMG)to graded colorectal distension. The pathological change in distal colon
                were observed by HE staining. The mRNA expressions of hypothalamic corticotropin releasing factor(CRF),corticotropin releasing
                factor receptor1(CRF1R),corticotropin releasing factor receptor 2(CRF2R),nesfatin⁃1,and colonic serotonin receptor 4(5⁃HT4R)
                were detected by quantitative real ⁃ time PCR. Results:Rats in MC group show higher EMG activity,and mRNA expression of


               [基金项目] 江苏省中医药科技项目(YB201789);江苏省“六大人才高峰”高层次人才项目(2016⁃WSN⁃094);江苏省第三批中
                医临床优秀人才研修项目
                ∗
                通信作者(Corresponding author),E⁃mail:chenhuan26384@163.com;z1356@126.com
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