Page 40 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第5期
·670 · Journal of Nanjing Medical University（Natural Sciences） 2021年5月

·基础医学·

Ag2S@BSA存储液对纯钛表面成骨细胞行为的影响

汤泽华，朱文卿，邱 憬 *

南京医科大学附属口腔医院种植科，江苏省口腔疾病研究重点实验室，江苏省口腔转化医学工程研究中心，江苏 南京 210029

［摘 要］ 目的：研究含牛血清白蛋白（bovine serum albumin，BSA）包裹的硫化银存储液对纯钛表面特性及成骨细胞生物学行
为的影响。方法：纯钛试件经打磨清洗后分为4组，分别置于空气、生理盐水、BSA溶液、牛血清白蛋白包裹的硫化银溶液（BSA⁃
coated Ag2S solution，Ag2S@BSA）中保存 2 周后，采用扫描电镜、X 线能谱仪和光学接触角仪分析纯钛表面特性的变化；将
MC3T3⁃E1成骨细胞接种于各组钛片表面，观察成骨细胞的黏附、增殖和分化行为。结果：扫描电镜和能谱分析结果显示，各组
钛表面微形貌无明显差异，空气存储后未见明显改变，经生理盐水存储后钛表面散在分布氯化钠晶体，经BSA溶液存储后含碳
有机物增加，经Ag2S@BSA存储后钛表面均匀吸附含硫和银元素的纳米颗粒。与其余3种存储方式相比，Ag2S@BSA溶液存储
可显著减小钛表面水接触角，增大表面能，显著促进成骨细胞的黏附、增殖和成骨功能蛋白表达。结论：Ag2S@BSA存储液能优
化钛表面特性，增强其成骨活性。
［关键词］ 牛血清白蛋白；Ag2S；存储液；钛；成骨细胞
［中图分类号］ R329.28 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）05⁃670⁃07
doi：10.7655/NYDXBNS20210506

Effects of a Ag2S@BSA ⁃ containing storage solution on osteoblasts behaviors on pure
titanium surface

TANG Zehua，ZHU Wenqing，QIU Jing *
Department of Oral Implantology，the Affiliated Stomatological Hospital of Nanjing Medical University；Jiangsu
Province Key Laboratory of Oral Diseases；Jiangsu Privince Engineering Research Center of Stomatological
Trarslational Medicine，Nanjing 210029，China

［Abstract］ Objective：This study aims to investigate the effects of bovine serum albumin（BSA）coated Ag2S storage solution on the
surface characteristics of pure titanium and the behaviors of osteoblasts. Methods：After polishing and cleaning，pure titanium disks
were stored in air，saline，BSA solution and BSA ⁃ coated Ag 2S storage solution（Ag2S@BSA）respectively for two weeks. Scanning
electron microscope（SEM），energy dispersive spectrometer（EDS）and contact angle test were used to analyze surface characteristics
of pure titanium preserved in four media. Assays of adhesion，proliferation，and differentiation of MC3T3⁃E1 cells were conducted to
evaluate the biocompatibility of different samples. Results：SEM and EDS results demonstrated that sodium chloride crystals were
scattered on titanium surface in the saline group，while carbon and oxygen distribution were denser in the BSA group. Nano⁃sized
particles containing silver and sulfur were adsorbed on the titanium surface without changing the original morphology. Compared with
the other three groups，titanium surfaces preserved in Ag 2S@BSA solution showed superior hydrophilicity and significantly promoted
the adhesion proliferation and differentiation abilities of osteoblasts with high expression of osteogenesis⁃related proteins. Conclusion：
The Ag2S@ BSA⁃containing storage solution can enhance the osteogenic activity of osteoblasts by improving the surface characteristics
of pure titanium.
［Key words］ BSA；Ag2S；storage solution；titanium；osteoblast
［J Nanjing Med Univ，2021，41（05）：670⁃676］

［基金项目］ 国家自然科学基金面上项目（81870799）；江苏省重点研发计划（社会发展）项目（BE2019728）；江苏省青年医学
人才项目（QNRC2016850）；南京医科大学⁃苏盐智创新研发基金项目（NMU⁃SY201806）；镇江市重点研发计划（社会发展）项目
（SH2019040）；江苏高校优势学科建设工程资助项目（2018⁃87）
∗
通信作者（Corresponding author），E⁃mail：qiujing@njmu.edu.cn