Page 26 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第41卷第6期
·804 · Journal of Nanjing Medical University（Natural Sciences） 2021年6月

·基础医学·

B10细胞在放射性肺纤维化进程中的动态变化

潘晓娴 ，王彩虹 ，王 锃 2，3，4 ，陈金梅 ，蓝瑞隆 2，3，4 ，陈俊英 2，3，4 ，张纬建 ，黄 菲 2，3，4* ，洪金省 1，2*
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1，2
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1，2
福建医科大学附属第一医院放疗科，福建 福州 350005；福建省高等学校放射生物重点实验室，福建 福州 350005；福
1 2 3
建省肿瘤精准诊疗重点实验室，福建 福州 350005；福建医科大学附属第一医院中心实验室，福建 福州 350005
4
［摘 要］ 目的：探究B10细胞放射性肺纤维化进程中的动态变化。方法：将30只6~8周C57BL/6小鼠随机分为未照射对照
组、照射后2 d组（IR2d组）、照射后14 d 组（IR14d组）、照射后3个月组（IR3m组）、照射后5个月组（IR5m组），应用电离辐射建
立放射性肺纤维化的动物模型，通过HE染色法、Masson染色观察肺组织的病理变化，免疫荧光染色法观察α⁃平滑肌肌动蛋白
（α⁃smooth muscle actin，α⁃SMA）表达，流式细胞术检测B10细胞在肺、脾脏中的数目变化。结果：胸部照射后早期小鼠肺组织
呈现炎性反应；照射后3个月出现肺泡结构破坏，间质填充，照射后5个月大量胶原填充，α⁃SMA表达较对照组明显增加。IR2d
组、IR14d组肺组织中的B10细胞比例较对照组明显增加，而IR3m组B10细胞较IR2d组明显下降；照射后2 d脾脏中B10细胞开始
增加，照射后14 d达到峰值，照射后3个月较照射后2 d、14 d明显下降。结论：放射性肺纤维化进程中存在B10细胞浸润，呈现早
期升高晚期降低的趋势。
［关键词］ B10细胞；肺纤维化；电离辐射
［中图分类号］ R563.9 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）06⁃804⁃06
doi：10.7655/NYDXBNS20210603

Dynamic changes of B10 cells in the process of radiation⁃induced pulmonary fibrosis

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PAN Xiaoxian ，WANG Caihong ，WANG Zheng 2，3，4 ，CHEN Jinmei ，LAN Ruilong 2，3，4 ，CHEN Junying 2，3，4 ，ZHANG
1，2
Weijian ，HUANG Fei 2，3，4* ，HONG Jinsheng 1，2*
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Department of Radiotherapy，the First Affiliated Hospital of Fujian Medical University，Fuzhou 350005；Key
Laboratory of Radiation Biology of Fujian Higher Education Institutions，Fuzhou 350005；Fujian Provincial Key
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Laboratory of Precision Medicine for Cancer，Fuzhou 350005；Central Lab，the First Affiliated Hospital of Fujian
Medical University，Fuzhou 350005，China
［Abstract］ Objective：This study aims to explore the dynamic changes in the process of radiation ⁃ induced pulmonary fibrosis
（RIPF）of B10 cells. Methods：30 C57BL/6 mice，6⁃8 weeks old，were randomly divided into non⁃irradiated（control）group，2 days
after irradiation（IR2d），14 days after irradiation（IR14d），3 month after irradiation（IR3m）and 5 month after irradiation（IR5m）group.
The radiation⁃induced pulmonary fibrosis model was established by using ionizing radiation. HE staining and Masson staining were
used to observe the pathological changes of lung tissue，and the expression of α⁃SMA was observed by immunohistochemistry. Flow
cytometry was used to detect the infiltration changes of B10 cells in lung tissues and spleens in the RIPF model. Results：The lung
tissues of mice showed inflammatory response in the early stage after chest irradiation. Furthermore，the alveolar structure destruction
and interstitial filling began to appear 3 months after irradiation，and a large amount of collagen was filled 5 months after irradiation.
The expression of α ⁃ SMA was significantly higher than that of the control group，and pulmonary fibrosis was more severe. The
proportion of B10 cells in lung tissue was significantly increased at 2 d and 14 d after chest irradiation compared with the non ⁃
irradiated group，while was significantly lower in IR3m group than in IR2d group，B10 cells in the spleens began to increase at IR2d，
and reached a peak at IR14d，and was significantly lower in IR3m group than in IR2d group and IR14d group. Conclusion：There were
B10 cells infiltration in the process of radioactive pulmonary fibrosis，with a trend of early increase and late decrease.
［Key words］ B10 cells；pulmonary fibrosis；ionizing radiation
［J Nanjing Med Univ，2021，41（06）：804⁃809］
［基金项目］ 国家自然科学基金面上项目（81872554）；福建省自然科学基金资助项目（2018J01169）
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通信作者（Corresponding author），E⁃mail：hjs703@126.com；feifeigood2148@126.com