Page 69 - 南京医科大学学报自然科学版

第41卷第7期 南京医科大学学报（自然科学版）
2021年7月 Journal of Nanjing Medical University（Natural Sciences） ·999 ·

·基础医学·

卵巢癌细胞生长环境中 CD4 Treg 细胞和 CD4 Teff 细胞的糖代
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谢特征初探

吴 茗，徐 睿，刘书娜，王 芳 *

南京医科大学第一附属医院检验学部，江苏 南京 210029

［摘 要］ 目的：探究 CD4 CD25 CD127 调节性 T 细胞（regulatory T cell，Treg）和 CD4 CD25 CD127 效应性 T 细胞（effector T
low
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cell，Teff）在卵巢癌细胞生长环境中的糖代谢特征。方法：流式细胞术（fluorescence⁃activated cell sorting，FACS）分选CD4 Treg
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细胞和 CD4 Teff 细胞，建立两种细胞与 SKOV3 细胞的共培养体系，采用实时荧光定量 PCR（real⁃time fluorescence quantitative
PCR，RT⁃PCR）和Western blot 检测CD4 Treg细胞、CD4 Teff 细胞糖代谢相关基因和蛋白的表达水平，比色法检测细胞糖摄取
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和糖酵解水平。结果：SKOV3共培养组CD4 Treg细胞糖代谢相关基因和蛋白的表达水平高于CD4 Teff细胞和CD4 Treg细胞
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单独培养组（P＜0.05）；SKOV3共培养组CD4 Treg细胞糖摄取和糖酵解水平高于CD4 Teff细胞［（59.78±2.57）pmol vs.（44.93±
1.86）pmol，P＜0.05；（15.31 ± 2.05）mmol/L vs.（7.98 ±0.88）mmol/L，P＜0.05］。结论：卵巢癌细胞生长环境中的人外周血 CD4 +
Treg细胞糖代谢水平高于CD4 Teff细胞，卵巢癌细胞可以促进人外周血CD4 Treg细胞糖代谢相关蛋白和基因表达水平上调。
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［关键词］ 卵巢癌；调节性T细胞；效应性T细胞；糖代谢
［中图分类号］ R737.31 ［文献标志码］ A ［文章编号］ 1007⁃4368（2021）07⁃999⁃07
doi：10.7655/NYDXBNS20210710
The glucose metabolism characteristics of CD4 Treg cells and CD4 Teff cells in growth
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environment of ovarian cancer cells
WU Ming，XU Rui，LIU Shuna，WANG Fang *
Department of Laboratory Medicine，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，
China
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［Abstract］ Objective：To investigate the glucose metabolism characteristics of CD4 CD25 CD127 low regulatory T cells（Treg）and
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CD4 CD25 CD127 effector T cells（Teff）in growth environment of ovarian cancer cells. Methods：Fluorescence⁃activated cell sorting
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（FACS）separated CD4 Treg cells and CD4 Teff cells，then established a coculture system of human T cells with ovarian cancer cell
SKOV3. The expression levels of glucose metabolism related genes and proteins were determined by real⁃time fluorescence quantitative
PCR（RT⁃PCR）and Western blot. The levels of glucose uptake and glycolysis were detected by colorimetry. Results：Compared with
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the CD4 Treg cells in single culture without SKOV3 and CD4 Teff cells，the expression levels of glucose metabolism related genes and
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proteins in CD4 Treg cells cocultured with SKOV3 were elevated（P＜0.05）and the levels of glucose uptake and glycolysis were
increased［（59.78±2.57）pmol vs.（44.93±1.86）pmol，P＜0.05；（15.31 ± 2.05）mmol/L vs.（7.98 ±0.88）mmol/L，P＜0.05］. Conclusion：
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The level of glucose metabolism in CD4 Treg cells is higher than CD4 Teff cells in ovarian cancer cell growth environment，ovarian
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cancer cells could promote the expression levels of glucose metabolism related genes and proteins in CD4 Treg cells.
［Key words］ ovarian cancer；regulatory T cells；effector T cells；glucose metabolism
［J Nanjing Med Univ，2021，41（07）：999⁃1005］

［基金项目］ 国家自然科学基金（81772779）；江苏省“六大人才高峰”资助项目（2015⁃WSN⁃034）；江苏省“六个一工程”拔尖人
才项目（LGY2017068）；江苏省重点人才基金（ZDRCA2016003）；江苏省实验诊断学重点实验室（ZDXKB2016005）
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通信作者（Corresponding author），E⁃mail：wangfang@njmu.edu.cn