Page 69 - 南京医科大学学报自然科学版

第42卷第6期 南京医科大学学报（自然科学版）
2022年6月 Journal of Nanjing Medical University（Natural Sciences） ·821 ·

·基础研究·

兔上颌前牙即刻种植动物模型的构建及其骨结合观察

叶笛笛，柳 姚，邱 憬 *
南京医科大学附属口腔医院种植科，江苏省口腔疾病研究重点实验室，江苏省口腔转化医学工程研究中心，江苏 南京
210029

［摘 要］ 目的：构建新西兰兔上颌前牙即刻种植的实验动物模型，观察不同时间点的种植体骨结合特征。方法：准备16只
新西兰兔，随机分为2周、4周、8周、12周组，每组4只。拔除兔的上颌前牙，在其新鲜拔牙窝内即刻植入纯钛实验种植体。术
后分别在2周、4周、8周、12周采集含种植体的上颌骨标本，再通过大体观察、Micro⁃CT扫描、组织切片染色研究即刻种植后不
同时间点的种植体骨结合特征。结果：①大体观察：各组实验兔的术后状态正常，食欲无明显异常，创口愈合良好，种植体均未
松动脱落。②Micro⁃CT 分析：随着种植体植入后愈合时间的延长，种植体螺纹处的骨质密度和厚度不断增加。③ 组织学检
测：随着种植体植入后时间的延长，硬组织切片染色测算所得的种植体⁃骨接触率（bone⁃implant contact ratio，BIC）稳步增长，而
脱钙颌骨切片染色显示种植体周围的骨质结构呈散在⁃岛状⁃条索状⁃板层状骨演化，提示骨结合形成良好。2周和4周组中存在大
量的破骨细胞和炎症细胞，8周和12周组中则几乎未见这类细胞，但存在更多的红细胞和黄骨髓。结论：兔上颌前牙拔除后即刻
植入种植体可构建即刻种植动物模型，为即刻种植后骨结合过程的观察以及钛种植体的动物体内研究提供新的选择和途径。
［关键词］ 新西兰兔；上颌前牙；即刻种植；动物模型；骨结合
［中图分类号］ R783.6 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）06⁃821⁃09
doi：10.7655/NYDXBNS20220609

Establishment of an animal model of immediate implantation for maxillary anterior teeth in
rabbits and its impact on osseointegration

YE Didi，LIU Yao，QIU Jing *
Department of Oral Implantology，Jiangsu Province Key Laboratory of Oral Diseases，Jiangsu Province Engineering
Research Center of Stomatological Trarslational Medicine，the Affiliated Stomatological Hospital of Nanjing Medical
University,Nanjing 210029，China

［Abstract］ Objective：This study aims to establish an animal model of immediate implantation for maxillary anterior teeth of New
Zealand rabbits and observe its osseointegration at different time points. Methods：A total of 16 New Zealand rabbits were randomly
divided into 4 groups according to 2 week，4 week，8 week and 12 week，with 4 rabbits in each group. After surgery，maxillary
specimens containing implants were collected at 2 week，4 week，8 week and 12 week，respectively. The characteristics of implant
osseointegration at different time points after immediate implantation were studied by observation，Micro ⁃ CT scanning and tissue
section staining. The maxillary anterior teeth of rabbits were extracted and pure titanium experimental implants were immediately
implanted in the fresh extraction sockets. Maxillary specimens containing implants were collected at 2，4，8 and 12 weeks
postoperatively，and the characteristics of implant osseointegration at different time points after immediate implantation were studied by
general observation，Micro⁃CT scanning，and staining of tissue sections. Results：①General observation：the rabbits in each group had
normal postoperative state，no obvious abnormality of appetite，good wound healing，and no loosening or falling off implants. ②Micro⁃
CT analysis：the bone density and thickness at the thread of implant increased continuously with the extension of healing time after
implantation. ③Histological examination：according to the time after implantation，the bone⁃implant contact ratio（BIC）measured by
staining of hard tissue sections increased steadily，and the bone structure around the implant，as shown in the staining of decalcified

［基金项目］ 国家自然科学基金面上项目（81870799）；江苏省重点研发计划（社会发展）项目（BE2019728）；江苏高校优势学
科建设工程资助项目（2018⁃87）
∗
通信作者（Corresponding author），E⁃mail：qiujing@njmu.edu.cn