Page 70 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第8期
·1112 · Journal of Nanjing Medical University（Natural Sciences） 2022年8月

·基础研究·

环状 RNA hsa_circ_0005579 在子痫前期患者胎盘中的表达及

对滋养层细胞增殖、迁移及侵袭的影响

高明明 ，俞兆儿 ，姚 丹 ，仲 红 ，贾瑞喆 1*
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南京医科大学附属妇产医院（南京市妇幼保健院）产科，医学研究中心，江苏 南京 210004
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［摘 要］目的：探究环状RNA hsa_circ_0005579作为子痫前期发展的新生物标志物的潜力，观察其在子痫前期患者胎盘中的
表达及其对滋养层细胞增殖、迁移及侵袭的影响。方法：通过实时定量聚合酶链式反应（qRT⁃PCR）检测20例子痫前期患者和
20例健康妊娠对照者胎盘组织中hsa_circ_0005579的表达，收集参与者的临床信息。采用核糖核酸酶R（RNase R）消化分析、
琼脂糖凝胶电泳及Sanger测序对hsa_circ_0005579进行鉴定，通过敲除siRNA转染HTR⁃8/SVneo细胞，利用CCK⁃8法、克隆形
成分析、EdU 实验检测hsa_circ_0005579对滋养层细胞增殖的影响，采用Transwell 分析、划痕实验检测hsa_circ_0005579对滋
养层细胞迁移及侵袭的影响。同时，蛋白质印迹（Western blot）分析用于检测迁移标志物的蛋白质水平。结果：hsa_circ_
0005579在子痫前期患者胎盘中有显著的高表达。此外，与si⁃NC组相比，干扰hsa_circ_0005579的表达细胞活力明显增强、克
隆形成数目增多、细胞增殖率增加、迁移及侵袭数目显著增多、细胞迁移率提高以及迁移标志物基质金属蛋白酶2（MMP2）、基
质金属蛋白酶 9（MMP9）蛋白水平提升。结论：环状 RNA hsa_circ_0005579 在子痫前期患者胎盘中高表达，沉默 hsa_circ_
0005579后HTR⁃8/SVneo的增殖、迁移及侵袭能力增强，有望为子痫前期的早期诊断和干预提供新的靶标。
［关键词］ 环状RNA；circ_0005579；滋养细胞；子痫前期

［中图分类号］ R714.245 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）08⁃1112⁃07
doi：10.7655/NYDXBNS20220810

The expression of circular RNA hsa_circ_0005579 in the placenta of patients with
preeclampsia and its effect on the proliferation，migration and invasion of trophoblast cells

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GAO Mingming ，YU Zhaoer ，YAO Dan ，ZHONG Hong ，JIA Ruizhe
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1 Department of Obstetrics，Department of Medical Research Center，Women’s Hospital of Nanjing Medical University，
Nanjing Maternity and Child Health Care Hospital，Nanjing 210004，China

［Abstract］ Objective：To explore the potential of the circular RNA hsa_circ_0005579 as a new biomarker for the development of pre
⁃eclampsia，and to observe its expression in the placenta of patients with pre⁃eclampsia and its effect on the proliferation，migration and
invasion of trophoblast cells. Methods：The expression of hsa_circ_0005579 was detected in placental tissues obtained from 20 women
with pre⁃eclampsia and 20 healthy pregnancy controls using real⁃time quantitative polymerase chain reaction（qRT⁃PCR）. Clinical
information of participants was collected. Ribonuclease R（RNase R）digestion analysis，agarose gel electrophoresis and Sanger
sequencing were used to verify hsa_circ_0005579. HTR⁃8/SVneo cells were transfected with hsa_circ_0005179 siRNA，cell counting
kit⁃8（CCK⁃8）assay，colony formation assay，EdU test were performed to detect the effect of hsa_circ_0005579 on the proliferation of
trophoblast cells. Transwell assay and wound healing assay were used to detect the effect of hsa_circ_0005579 on the migration and
invasion of trophoblast cells. Meanwhile，Western blot analysis was used to detect the protein level of migration markers. Results：
Hsa_circ_0005579 had a significant high expression in the placenta of PE patients. In addition，compared with the si⁃NC group，the
cell viability was significantly increased，the number of clone formation grew，the cell proliferation rate increased，and the number of
migration and invasion did rise，the cell migration rate was upregulated，and the protein levels of migration markers matrix
metalloproteinase 2（MMP2）and matrix metalloproteinase 9（MMP9）was enhanced in the si⁃circ_0005579 group. Conclusion：Circular

［基金项目］ 国家自然科学基金（81971393）
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通信作者（Corresponding author），E⁃mail：jiaruizhe2016@163.com