Page 22 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第42卷第9期
·1216 · Journal of Nanjing Medical University（Natural Sciences） 2022年9月

·基础研究·

BMP⁃2缓释型PLGA微囊作为引导骨再生支架的初步研究

王 莹 1，2，3 ，陈 晨 1，2，3* ，陈 刚 2，3，4*
南京医科大学附属口腔医院牙体牙髓科，江苏 南京 210029；南京医科大学口腔疾病研究江苏省重点实验室，江苏 南
1 2
京 210029；江苏省口腔转化医学工程研究中心，江苏 南京 210029；南京医科大学附属口腔医院口腔修复科，江苏 南
3
4
京 210029

［摘 要］ 目的：探索双通道同轴微量注射法制备聚乳酸⁃聚乙醇酸共聚物［poly（lactic⁃co⁃glycolic acid），PLGA］微囊用于引导
骨再生。方法：用双通道微量注射泵制备包封骨形态发生蛋白2（bone morphogenetic protein 2，BMP⁃2）的PLGA微囊。用光学
显微镜、荧光显微镜及扫描电子显微镜（scanning electron microscopy，SEM）表征微囊形态及结构；PBS浸泡法表征微囊降解性
能；CCK⁃8法及细胞Calcein/PI染色检测微囊细胞相容性；Transwell实验检测包封BMP⁃2的微囊作用24 h对MC3T3⁃E1细胞的
趋化作用。结果：制备的PLGA微囊为规则且单一囊腔的球形，直径167.58 μm，可获得两种囊壁平均厚度［（23.82±7.10）μm和
（35.31±5.55）μm］不同的微囊；微囊2个月内基本降解，CCK⁃8及细胞染色结果显示微囊细胞相容性好；Transwell实验证明微囊
作用24 h即对细胞有趋化作用。结论：本研究制备的微囊可通过控制囊壁厚度来控制因子缓释，其表面利于细胞黏附，有望成
为一种新型引导骨再生的支架材料。
［关键词］ PLGA微囊；引导骨再生；BMP⁃2；可控缓释
［中图分类号］ R783.1 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）09⁃1216⁃07
doi：10.7655/NYDXBNS20220903

Preliminary study of a BMP ⁃ 2 releasing scaffold comprised of PLGA microspheres for
guiding bone regeneration

1，2，3 1，2，3* 2，3，4*
WANG Ying ，CHEN Chen ，CHEN Gang
Department of Endodontics，the Affiliated Stomatological Hospital of Nanjing Medical University，Nanjing 210029；
1
2 Jiangsu Province Key Laboratory of Oral Diseases，Nanjing Medical University，Nanjing 210029；Jiangsu Province
3
4
Engineering Research Center of Stomatology Translation Medicine，Nanjing 210029；Department of Prosthodontics，
the Affiliated Stomatological Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：This study invented a coaxial dual⁃channel micro⁃injection technique to prepare the poly（lactic⁃co⁃glycolic
acid）（PLGA）microspheres for the first time，which can be used for guiding bone regeneration. Methods：A dual⁃channel microsyringe
was used to prepare PLGA microspheres encapsulated bone morphogenetic protein 2（BMP⁃2）. An optical microscope，a fluorescence
microscopy，a scanning electron microscopy（SEM）were used to observe morphology and structure of the microspheres. The changes of
morphology and mass loss of the microspheres soaked in PBS solution were recorded for degradation. Cytocompatibility of the
microspheres was detected by CCK⁃8 and cell Calcein/PI staining kit. The effect of PLGA microspheres loading with BMP⁃2 on the
chemotaxis of MC3T3⁃E1 cell in 24 hours was detected by Transwell cell migration assay. Results：The microspheres in this study
showed regular mono cavity. The mean diameter of the microspheres was 167.58 μm. Two different mean thickness of microsphere shell
［（23.82±7.10）μm and（35.31±5.55）μm］can be acquired. The microspheres degraded gradually in 2 months in PBS solution. The
results of CCK⁃8 and ceu Calcein/PI staining test showed cells remained activity with PLGA microspheres. Transwell cell migration
assay indicated PLGA microspheres loading with BMP⁃2 had chemotactic effect on cells in 24 hours. Conclusion：The microspheres in
this study with releasing rate of BMP⁃2 controlled by the thickness of microspheres shell and its surface adhered by cells easily，have a

［基金项目］ 国家自然科学基金（81970927）；江苏省自然科学基金（BK20191348）；江苏高校优势学科建设工程资助项目
（2018-87）
∗
通信作者（Corresponding author），E⁃mail：ccchicy@njmu.edu.cn；dchengangd@163.com