Page 29 - 南京医科大学学报自然科学版

第42卷第9期 南京医科大学学报（自然科学版）
2022年9月 Journal of Nanjing Medical University（Natural Sciences） ·1223 ·

·基础研究·

脑胶质瘤靶向SPIO⁃PLA⁃P53分子探针的构建及磁共振成像实

验研究

段丽珍，郭莉莉 ，柏根基
*
南京医科大学附属淮安第一医院影像中心，江苏 淮安 223300

［摘 要］ 目的：探讨靶向磁共振SPIO⁃PLA⁃P53分子探针对大鼠脑胶质瘤的诊断效能。方法：合成超顺磁性氧化铁纳米粒子
SPIO⁃PLA和脑胶质瘤靶向纳米分子探针SPIO⁃PLA⁃P53。使用透射电镜和动态光散射检测分子探针的物理性质；CCK⁃8（Cell
Counting Kit⁃8）法检测细胞活性；并检测分子探针体外透过血脑屏障的效能。利用7.0 T小动物专用磁共振仪及配套线圈检测
纳米探针在胶质瘤中的分布。对荷瘤鼠脑组织进行病理染色，明确靶向分子探针在肿瘤组织中的蓄积。使用GraphPad Prism
软件对结果进行统计学分析。结果：脑胶质瘤靶向SPIO⁃PLA⁃P53纳米粒子大小均匀，分散度较好，具有良好的生物相容性及
靶向聚集效应。磁共振扫描结果显示荷瘤大鼠在注射靶向SPIO⁃PLA⁃P53探针后瘤体T2WI信号减低，在注射SPIO⁃PLA探针
溶液后瘤体信号无明显变化。病理结果证实注射SPIO⁃PLA⁃P53大鼠胶质瘤内大量铁离子蓄积。结论：SPIO⁃PLA⁃53可作为大
鼠脑胶质瘤特异性显像的磁共振分子探针。
［关键词］ 脑胶质瘤；SPIO；磁共振成像；分子探针；P53
［中图分类号］ R739.41 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）09⁃1223⁃07
doi：10.7655/NYDXBNS20220904

Preparation of targeting SPIO ⁃ PLA ⁃ P53 glioma molecular probe and its application in
magnetic resonance imaging
*
DUAN Lizhen，GUO Lili ，BO Genji
Department of Medical Imaging，the Affiliated Huai’an No.1 People's Hospital of Nanjing Medical University，
Huai'an 223300，China

［Abstract］ Objective：This study aims to investigate the diagnostic efficacy of targeted magnetic resonance SPIO ⁃ PLA ⁃ P53
molecular probe in rat glioma. Methods：Superparamagnetic iron oxide nanoparticles SPIO⁃PLA and glioma targeting nanomolecular
probe SPIO⁃PLA⁃P53 were synthesized. The physical properties of molecular probes were detected by transmission electron microscopy
and dynamic light scattering. Cell activity was detected by Cell Counting Kit ⁃ 8（CCK ⁃ 8）method. The penetration efficiency of
molecular probe through blood⁃brain barrier in vitro was detected. The distribution of nanoprobes in glioma was detected by 7.0 T small
animal ⁃ special magnetic resonance instrument and matching coil. Pathological staining of tumor ⁃ bearing mouse brain tissue was
performed to clarify the accumulation of targeted molecular probes in the tumor tissue. Statistical analysis of the results was performed
using GraphPad Prism software. Results：Glioma targeted SPIO ⁃ PLA ⁃ P53 nanoparticles have uniform size，good dispersion，good
biocompatibility and targeted aggregation effect. Magnetic resonance scanning showed that the tumor T2WI signal was decreased after
injection of targeted SPIO⁃PLA⁃P53 probe in the tumor⁃bearing rats，and there was no significant change in the tumor signal after
injection of SPIO⁃PLA probe solution. The pathological results confirmed that a large amount of iron ions accumulated in the glioma of
rat injected with SPIO⁃PLA⁃P53. Conclusion：SPIO⁃PLA⁃P53 can be used as an magnetic resonance molecular probe for specific
imaging of rat gliomas.
［Key words］ glioma；SPIO；MRI；molecular probe；P53
［J Nanjing Med Univ，2022，42（09）：1223⁃1229］
［基金项目］ 南京医科大学科技发展基金基金（2016NJMU ZD085）
∗
通信作者（Corresponding author），E⁃mail：guolili163@163.com