Page 85 - 南京医科大学学报自然科学版

第42卷第9期 南京医科大学学报（自然科学版）
2022年9月 Journal of Nanjing Medical University（Natural Sciences） ·1279 ·

·临床研究·

外周血 Circ_0070934/miR⁃199a⁃5p/MGAT3 作为生物标志物在

过敏性鼻炎⁃哮喘综合征中的诊断价值

胡钰洁，吴 迪，黄燕华，刘志光，施宇佳，张 倩 *

南京医科大学附属常州第二人民医院呼吸与危重症医学科，江苏 常州 213003

［摘 要］ 目的：评估外周血circ_0070934/miR⁃199a⁃5p/MGAT3在过敏性鼻炎⁃哮喘综合征（combined allergic rhinitis and asthma
syndrome，CARAS）中的诊断价值。方法：收集CARAS患者38例和健康对照43例，采集外周静脉血，采用ELISA法检测血浆中
MGAT3蛋白水平，采用qRT⁃PCR 检测外周血中circ_0070934/miR⁃199a⁃5p/MGAT3的表达水平。ROC曲线判断指标的诊断灵
敏度与特异度。结果：ELISA结果显示，CARAS患者中MGAT3的浓度较对照组明显下降（P=0.035）。qRT⁃PCR结果显示，circ_
0070934（P=0.001）与 MGAT3（P < 0.001）的表达较对照组下调，而 miR⁃199a⁃5p 的表达水平上调（P=0.013）。相关性分析结果
显示，MGAT3 与嗜酸性粒细胞直接计数、嗜酸性粒细胞百分比呈正相关；circ_0070934 与二者呈负相关。ROC 曲线分析，
MGAT3 为最佳参数，取界值为 0.208 时，其灵敏度和特异度分别为 73.7%和 100.0%，AUC 为 0.933（95%CI：0.882~0.984，P <
0.001）；circ_0070934、miR⁃199a⁃5p和MGAT3 3个指标联合时，对CARAS的诊断价值最高，取界值为0.539时，灵敏度为92.1%，
特异度为 93.0%，AUC 为 0.959（95%CI：0.916~1.000，P < 0.001）。结论：CARAS 患者外周血 circ_0070934/miR⁃199a⁃5p/MGAT3
的表达存在差异，可作为诊断CARAS的生物标志物。进一步研究circ_0070934/miR⁃199a⁃5p/MGAT3在CARAS中的具体作用
和机制，有助于发现CARAS新的诊治靶点。
［关键词］ 过敏性鼻炎⁃哮喘综合征；circ_0070934；miR⁃199a⁃5p；MGAT3；生物标志物
［中图分类号］ R562.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2022）09⁃1279⁃08
doi：10.7655/NYDXBNS20220913

Diagnostic value of circ_0070934/mir⁃199a⁃5p/MGAT3 in peripheral blood as a biomarker
in combined allergic rhinitis and asthma syndrome

HU Yujie，WU Di，HUANG Yanhua，LIU Zhiguang，SHI Yujia，ZHANG Qian *
Department of Respiratory and Critical Care Medicine，the Affiliated Changzhou NO.2 People′s Hospital of Nanjing
Medical University，Changzhou 213003，China

［Abstract］ Objective：This study aims to evaluate the diagnostic value of peripheral blood circ_0070934/miR⁃199a⁃5p/MGAT3 in
combined allergic rhinitis and asthma syndrome（CARAS）. Methods：Peripheral venous blood was collected from 38 CARAS patients
and 43 healthy controls. Plasma MGAT3 protein level was detected by ELISA，and the expression level of circ_0070934/miR⁃199a⁃5p/
MGAT3 in peripheral blood was detected by quantitative real ⁃ time PCR（qRT ⁃ PCR）. Diagnostic sensitivity and specificity were
determined by ROC curve. Results：ELISA results showed that MGAT3 concentration in CARAS patients was significantly lower than
that in control group（P=0.035）. The circ_0070934（P=0.001）and MGAT3 mRNA expressions（P < 0.001）in CARAS patients were
down⁃regulated when compared with control group，while miR⁃199a⁃5p was up⁃regulated（P=0.013）. Correlation analysis showed that
MGAT3 was positively correlated with the count of eosinophils and the percentage of eosinophils，while circ_0070934 showed a
negative correlation. ROC curve analysis indicated that MGAT3 was the best parameter. When the cut ⁃ off value was 0.208，the
sensitivity and specificity were 73.7% and 100.0%，respectively，and the AUC was 0.933（95%CI：0.882~0.984，P < 0.001）. The
combination of circ_0070934，miR⁃199a⁃5p and MGAT3 had the highest diagnostic value for CARAS. When the cut⁃off value was

［基金项目］ 江苏省社会发展项目（BE2020651）；江苏省第五期“333”高层次人才项目（BRA2020015）；常州市科技项目
（CE20205023）
∗
通信作者（Corresponding author），E⁃mail：qianzhang@njmu.edu.cn