Page 22 - 南京医科大学学报自然科学版

南京医科大学学报（自然科学版） 第43卷第2期
·164 · Journal of Nanjing Medical University（Natural Sciences） 2023年2月

·基础医学·

大鼠心肌纤维化模型MR T2WI成像的实验研究

陆文烨，宋梦星，吴 芬，夏 敏，朱晓梅，马占龙 *
南京医科大学第一附属医院放射科，江苏 南京 210029

［摘 要］ 目的：应用心脏磁共振成像技术（cardiac magnetic resonance imaging，CMR）分析大鼠心肌纤维化模型的影像学特
征，探索心肌纤维化MR T2WI成像表现及病理学改变的相关性。方法：选取50只SD大鼠，随机选取40只皮下注射5 mg/kg异
丙肾上腺素10 d建立心肌纤维化模型，10只皮下注射同剂量生理盐水10 d作为对照组。造模成功后，模型组分为3组，分别于
第 5、7、9 天应用 7.0 T 磁共振设备，进行活体成像扫描，对照组于第 7 天进行扫描，并计算对比信噪比（contrast noise ratio，
CNR）。扫描完成后处死大鼠进行 HE 及 Masson 染色，观察心肌组织结构变化并计算胶原容积分数（collagen volume fraction，
CVF）。结果：模型组36只，对照组10只纳入统计学分析，磁共振显示5 d模型组磁共振图像CNR（5.84±1.28），7 d模型组CNR
（15.26±1.93），9 d模型组CNR（9.37±1.67），与对照组（3.56±0.93）差异具有统计学意义（P＜0.01）。MR T2WI成像7 d模型组与
对照组相比，左心室腔扩大，左侧心室壁呈T2WI低信号改变，心室壁变薄、厚度减少，与对照组左心室壁厚度为（2.73±0.23）mm
相比，模型组（1.19±0.29）mm 显著减少（P＜0.01），表明心室壁纤维化改变。对照组 CVF［（6.18±2.90）%］与模型组［（43.06±
6.59）%］比较差异有统计学意义（P＜0.01）。模型组CNR和CVF之间的相关系数值为0.993，P＜0.01，呈显著正相关。结论：大
鼠心肌纤维化模型的MR T2WI成像具有明显的病理学相关影像学特征，可用来开展心肌纤维化的影像学及临床基础研究。
［关键词］ 心肌纤维化；核磁成像；大鼠

［中图分类号］ R445.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）02⁃164⁃05
doi：10.7655/NYDXBNS20230203

Experimental study on MR T2WI imaging of the rat myocardial fibrosis model

LU Wenye，SONG Mengxing，WU Fen，XIA Min，ZHU Xiaomei，MA Zhanlong *
Department of Radiology，the First Affiliated Hospital of Nanjing Medical University，Nanjing 210029，China

［Abstract］ Objective：Cardiac magnetic resonance imaging（CMR）was used to analyze the imaging features of the rat model of
myocardial fibrosis，and to explore the correlation between MR T2WI findings and pathological changes of myocardial fibrosis.
Methods：Fifty SD rats were randomly selected，forty mice were randomly selected and subcutaneously injected with five mg/kg
isoproterenol for ten days to establish the myocardial fibrosis model，and ten rats were subcutaneously injected with the same dose of
normal saline for ten days as the control group. The model group was divided into three subgroups，and in vivo imaging scan was
performed on day five，seven，and nine with 7.0 T magnetic resonance equipment，respectively. The control group was scanned on day
seven and the contrast noise ratio（CNR）was calculated. The HE and Masson staining were performed to observe the structural changes
of myocardial tissues and calculate the collagen volume fraction（CVF）. Results：36 rats in the model group and 10 rats in the control
group were included in statistical analysis. The CNR of the model group（15.26 ± 1.93）and the control group（3.56 ± 0.93）showed
statistically significant differences（P＜0.01）. Compared with the control group，the left ventricular lumen of the model group was
enlarged，the left ventricular wall showed T2WI hyposignal changes，and the thickness of the ventricular wall decreased and became
thinner. The left ventricular wall thickness of the control group was（2.73±0.23）mm，which was statistically significant compared with
that of the model group（1.19±0.29）mm（P < 0.01）. These indicated fibrotic changes in the ventricular wall.The CVF of the control
group（6.18% ± 2.90%）was significantly different from that of the model group（43.06% ± 6.59%）（P < 0.01）. The value of phase
relationship between CNR and CVF was 0.993，showing a significant positive correlation. Conclusion：The MR T2WI of the myocardial
fibrosis model of rats is characterized by obvious pathological imaging characteristics，which could be used to carry out imaging and
clinical research of myocardial fibrosis.
［Key words］ myocardial fibrosis；MRI；rat
［基金项目］ 国家自然科学基金（81971669） ［J Nanjing Med Univ，2023，43（02）：164⁃168］
∗
通信作者（Corresponding author），E⁃mail：mazhanlong@126.com