Page 29 - 南京医科大学学报自然科学版

第43卷第3期 南京医科大学学报（自然科学版）
2023年3月 Journal of Nanjing Medical University（Natural Sciences） ·319 ·

·基础研究·

CCT2对肺腺癌细胞增殖、迁移的影响及其临床意义

何树燕，沈 冬，林青凤，陈 洁，马成龙 *
南通大学附属江阴医院肿瘤科，江苏 江阴 214400

［摘 要］ 目的：探讨CCT2（chaperonin containning TCP1，subunit 2）在人肺腺癌中的表达、对肺腺癌细胞增殖、迁移生物学功
能的影响及其与患者临床特征的关系。方法：通过TCGA和GTEx数据库分析在肺腺癌组织及正常肺组织样本中CCT2的表达
差异，生存分析评价其表达水平与患者预后的关系。免疫组织化学法分别检测 CCT2、Ki⁃67 在 72 例肺腺癌组织中的表达水
平，并分析其表达水平与临床病理特征之间的相关性。采用逆转录⁃聚合酶链反应（qRT⁃PCR）、Western blot检测CCT2在肺腺
癌细胞中的表达情况；A549、H1650、H1299细胞株分别转染siRNA，分为si⁃NC 组和si⁃CCT2组，qRT⁃PCR、Western blot 验证转
染效率，CCK⁃8实验检测肺腺癌细胞增殖能力，细胞划痕实验检测细胞迁移能力。结果：基于TCGA数据库的肺腺癌数据分析
表明，与正常肺组织相比，肺腺癌组织中 CCT2 的表达水平更高，CCT2 的高表达与肺腺癌患者的总体生存率降低相关（P＜
0.05）。qRT⁃PCR、Western blot检测显示，CCT2在肺腺癌细胞中高表达，免疫组化显示CCT2的表达与Ki⁃67表达呈正相关（P＜
0.05）。CCK⁃8实验检测结果显示，A549、H1650细胞在转染siRNA 48 h后，相比si⁃NC组，si⁃CCT2组细胞增殖能力降低，差异有
统计学意义（P＜0.05）。细胞划痕实验结果显示，A549、H1650细胞在转染siRNA 24 h后，si⁃CCT2组细胞的迁移率低于si⁃NC
组，差异均有统计学意义（P＜0.05）。结论：CCT2在肺腺癌细胞和组织中表达水平增加，敲低其表达可减弱A549、H1650肺腺
癌细胞的增殖及迁移。
［关键词］ 肺腺癌；CCT2；增殖；迁移
［中图分类号］ R734.2 ［文献标志码］ A ［文章编号］ 1007⁃4368（2023）03⁃319⁃07
doi：10.7655/NYDXBNS20230304

Effects of CCT2 on the proliferation and migration of lung adenocarcinoma cells and its
clinical significance

HE Shuyan，SHEN Dong，LIN Qingfeng，CHEN Jie，MA Chenglong *
Department of Oncology，Jiangyin Hospital Affiliated to Nantong University，Jiangyin 214400，China

［Abstract］ Objective：The current study aims to explore the biological function of CCT2（chaperonin containning TCP1，subunit 2）
in human lung adenocarcinoma. Methods：The expression divergence of CCT2 in lung adenocarcinoma tissues and normal lung tissue
samples were analyzed from TCGA and GTEx database. The relationship between the expression level of CCT2 and the patient’s
prognosis was evaluated through survival analysis. The expression of CCT2 in lung adenocarcinoma cells was measured by Reverse
Transcription⁃Polymerase Chain Reaction（qRT⁃PCR）and Western blot. CCT2 siRNA was transfected into cell strains（A549，H1650 or
H1299）respectively，which were divided into Group si ⁃ NC（negative control group）and Group si ⁃ CCT2（transfection group）.
Transfection efficiency was detected by qRT⁃PCR and Western blot. The proliferation ability of lung adenocarcinoma cell was tested by
CCK⁃8. The cellular migration ability was detected by wound⁃healing experiment. The expression levels of CCT2 and Ki⁃67 in 72 cases
of lung adenocarcinoma tissues were detected with immunohistochemical method，and the correlation between their expression levels
and clinical pathology features were analyzed. Results：The results showed that，compared with normal lung tissues，the expression
level of CCT2 in lung adenocarcinoma tissue was higher，which was related to low overall survival rate of lung adenocarcinoma patients
（P＜0.05）. According to qPCR and Western blot test，CCT2 highly expressed in lung adenocarcinoma cell，and the
immunohistochemistry result showed that the CCT2 expression was positively correlated with the Ki⁃67 expression（P＜0.05）. CCK⁃8 test

［基金项目］ 江苏省卫生健康委员会面上项目（H2019103）；江阴市卫健委面上项目（S202101）；江阴市卫健委面上项目
（S202003）
∗
通信作者（Corresponding author），E⁃mail：machenglong2018@foxmail.com