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南京医科大学学报(自然科学版) 第44卷第1期
· 12 · Journal of Nanjing Medical University(Natural Sciences) 2024年1月
·基础研究·
间歇性缺氧大鼠早期动脉粥样硬化中血管生成性T淋巴细胞的
表达变化
孙建宇,郭 华,张云云,崔小川 *
南京医科大学附属无锡人民医院全科医学科,江苏 无锡 214023
[摘 要] 目的:探讨慢性间歇性缺氧(chronic intermittent hypoxia,CIH)暴露下,发生早期动脉粥样硬化血管病变时,大鼠外
周血中血管生成性T淋巴细胞(angiogenic T lymphocyte,Tang)水平的变化。方法:将健康成年Sprague Dawley(SD)大鼠随机分
为2组:正常饲养组(RA组)和慢性间歇性缺氧组(IH组),其中IH组接受间歇性缺氧暴露,每天8 h,共6周。间歇性缺氧模式
设定为:每2 min 1个循环,40 s内向仓内充入氮气使氧气浓度从21%降到8%并稳定20 s,然后充入正常空气使氧气浓度在40 s
内恢复到21%并维持20 s。造模完成后,使用流式细胞法检测Tang及内皮祖细胞(endothelial progenitor cell,EPC)水平;ELISA
法检测高密度脂蛋白胆固醇(high⁃density lipoprotein cholesterol,HDL⁃C)、低密度脂蛋白胆固醇(low⁃density lipoprotein cholesterol,
LDL⁃C)、总胆固醇(total cholesterol,TC)、甘油三酯(triglycerides,TG)及同型半胱氨酸(homocysteine,Hcy);HE染色观察胸主动
脉病理改变并测量动脉内中膜厚度(intima⁃media thickness,IMT)。结果:流式结果显示,与RA组相比,IH组小鼠Tang水平显
著升高[(22.975±1.866)% vs.(15.713±1.746)%,P < 0.001],EPC水平差异无统计学意义[(0.040±0.028)% vs.(0.028±0.012)%,
P > 0.05];HE染色结果显示,IH组小鼠IMT比RA组显著增厚[(121.275±30.896)μm vs.(84.075±7.452)μm,P < 0.05];ELISA
实验表明,与RA组比,IH组Hcy水平显著升高[(6.2±0.6)μmol/L vs.(5.5±0.6)μmol/L,P < 0.05],但HDL⁃C、LDL⁃C、TC、TG水
平差异无统计学意义。结论:CIH大鼠血管发生了动脉粥样硬化早期病变,同时Tang细胞水平升高,提示Tang细胞水平与早
期动脉粥样硬化的发生发展有关。
[关键词] 血管生成性T淋巴细胞;慢性间歇性缺氧;动脉粥样硬化
[中图分类号] R543.5 [文献标志码] A [文章编号] 1007⁃4368(2024)01⁃012⁃05
doi:10.7655/NYDXBNSN230636
Changes in angiogenic T lymphocytes under early atherosclerosis in rats exposed to chronic
intermittent hypoxia
*
SUN Jianyu,GUO Hua,ZHANG Yunyun,CUI Xiaochuan
Department of General Practice,the Affiliated Wuxi People’s Hospital of Nanjing Medical University,Wuxi 214023,
China
[Abstract] Objective:To explore the changes in levels of angiogenic T lymphocyte(Tang)in the peripheral blood of rats exposed to
chronic intermittent hypoxia(CIH)during early atherosclerotic vascular changes. Methods:Healthy adult Sprague Dawley(SD)rats
were randomly divided into two groups:the normal feeding group(RA group)and the chronic intermittent hypoxia group(IH group),
with the IH exposed to intermittent hypoxia for 8 h per day for a total of 6 weeks. The intermittent hypoxia pattern was set to repeat
every 2 min,with 40 s of nitrogen gas to reduce the oxygen concentration from 21% to 8% and stabilize for 20 s,followed by normal air
to restore the oxygen concentration to 21% for 40 s and maintain for 20 s. After modeling,levels of Tang and endothelial progenitor cells
(EPC)were measured by flow cytometry. Levels of high⁃density lipoprotein cholesterol(HDL⁃C),low⁃density lipoprotein cholesterol
(LDL⁃C),triglycerides(TG),total cholesterol(TC),and homocysteine(Hcy)were measured by ELISA. pathological changes were
observed in HE stained pathological sections of the thoracic aorta. Results:Flow cytometry results showed that compared to the RA
group,the levels of Tang in rats of the IH group were significantly elevated[(22.975±1.866)% vs.(15.713±1.746)%,P < 0.001],and
[基金项目] 国家自然科学基金(82200106)
∗
通信作者(Corresponding author),E⁃mail:cuixiaochuan@njmu.edu.cn