Page 29 - 南京医科大学学报自然科学版

第44卷第2期 袁秀琛，范世杰，段伟豪，等. 负载仙茅苷的3D打印复合支架促进血管化和成骨效应的研究［J］.
2024年2月 南京医科大学学报（自然科学版），2024，44（2）：162-169 ·167 ·

A B
Blank HGS HGSC
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2.5 Blank *
（ % ） 2.0 HGS
Cell viability 1.0 DAPI
HGSC
1.5
0.5
0.0
1 d 3 d 7 d

EdU

Merged

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A：CCK⁃8 assay for evaluating the effect of scaffold on BMSC cytotoxicity. P < 0.05（n=3）.B：EdU fluorescent staining for evaluating the effect of
scaffold on BMSC proliferation（×200）.
图4 支架的细胞毒性和增殖特性评价
Figure 4 Evaluation of cytotoxicity and proliferation properties of scaffolds

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B
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C D E
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Number of migration 100 Number of junction points 150 Number of branches 100

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0 0 0
Blank HGS HGSC Blank HGS HGSC Blank HGS HGSC
A：Evaluation of cell migration ability by transwell assay（×100）.B：Assessment of scaffold for cell tube formation ability（×200）.C：Quantitative
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***
analysis of cell migration count. D：Quantitative analysis of junction points count. E：Quantitative analysis of branch numbers. P < 0.05， P < 0.001（n=
3）.
图5 评价支架对HUVEC的迁移能力和细胞成管的效果
Figure 5 Evaluation of the scaffold’s effect on HUVEC migration and cell tube formation