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A B 1.0 C
BIBO3304
Control ISO BIBO3304+ISO β⁃catenin/GAPDH 0.8 ** ## 1.0 *

active β⁃catenin 92 kDa 0.6 p⁃GSK3β/t⁃GSK3β 1.5 #
0.4
p⁃GSK3β（Ser） 46 kDa 0.5
active 0.2 0 0
t⁃GSK3β 46 kDa
BIBO3304
Control BIBO3304+ISO Control BIBO3304+ISO
BIBO3304
GAPDH 37 kDa ISO ISO
A：The protein expressions of active β⁃catenin，p⁃GSK3β and t⁃GSK3β in the myocardial tissue of mice detected by Western blot. B：Quantitative
analysis of active β⁃catenin protein expression in each group. C：Quantitative analysis of p⁃GSK3β/t⁃GSK3β in each group. Compared with the control
##
#
**
*
group，P < 0.05，P < 0.01；compared with the ISO group，P < 0.05，P < 0.01（n=4）.
图4 BIBO3304抑制ISO诱导的β⁃catenin信号通路蛋白表达
Figure 4 BIBO3304 inhibited ISO⁃induced protein expressions of β⁃catenin pathway
A B C
［Leu31，Pro34］⁃NPY 1.0
Control 10 nmol/L 100 nmol/L 0.8 * * 0.8 *
active β⁃catenin 92 kDa β⁃catenin/GAPDH 0.6 0.6 *
p⁃GSK3β（Ser） 46 kDa 0.4 p⁃GSK3β/t⁃GSK3β 1.0
0.4
t⁃GSK3β 46 kDa 0.2
active 0.2
37 kDa
GAPDH 0 0
Control Pro34 ］ ⁃NPY Pro34 ］ ⁃NPY Control Pro34 ］ ⁃NPY Pro34 ］ ⁃NPY
（ 100 nmol/L）
（ 100 nmol/L）
（ 10 nmol/L）
（ 10 nmol/L）
［ Leu31， ［ Leu31， ［ Leu31， ［ Leu31，
D β⁃catenin DAPI Merge

Control

⁃NPY
Pro34 ］

［ Leu31，

⁃NPY
Pro34 ］ +BIBO3304

［ Leu31，

A：The protein expressions of active β⁃catenin，p⁃GSK3β and t⁃GSK3β in H9C2 cells detected by Western blot. B：Quantitative analysis of active β
*
⁃catenin protein expression in each group. C：Quantitative analysis of p⁃GSK3β/t⁃GSK3β in each group. Compared with the control group，P < 0.05（n=
3）. D：Nuclear β⁃catenin in the H9C2 cells analyzed by immunofluorescence staining（Bar=100 μm）.
图5 ［Leu31，Pro34］⁃NPY激活H9C2细胞的β⁃catenin信号通路
Figure 5 ［Leu31，Pro34］⁃NPY activated the β⁃catenin pathway in H9C2 cells